Single cell enzyme diagnosis on the chip

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  • Sissel Juul Jensen
  • ,
  • Charlotte Harmsen
  • ,
  • Mette Juul Nielsen, Aarhus University, Denmark
  • Magnus Stougaard
  • Kam W Leong, Duke University, United States
  • Birgitta R Knudsen
  • Yi-Ping Ho, Denmark
Conventional diagnosis based on ensemble measurements often overlooks the variation among cells. Here, we present a droplet-microfluidics based platform to investigate single cell activities. Adopting a previously developed isothermal rolling circle amplification-based assay, we demonstrate detection of enzymatic activities down to the single cell level with small quantities of biological samples, which outcompetes existing techniques. Such a system, capable of resolving single cell activities, will ultimately have clinical applications in diagnosis, prediction of drug response and treatment evaluation, as well as fundamental impact on the understanding of disease mechanisms
Original languageEnglish
JournalInternational Conference on Micro Electro Mechanical Systems
Pages (from-to)911 - 914
Number of pages4
Publication statusPublished - 20 Jan 2013
EventMicro Electro Mechanical Systems (MEMS), 2013 IEEE 26th International Conference on - Taipei, Taiwan
Duration: 20 Jan 201324 Jan 2013


ConferenceMicro Electro Mechanical Systems (MEMS), 2013 IEEE 26th International Conference on

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