Single-Cell Analysis Reveals That CD47 mRNA Expression Correlates with Immune Cell Activation, Antiviral Isgs, and Cytotoxicity

Lamin B. Cham; Miriam Rosas-Umbert; Lin Lin; Martin Tolstrup; Ole S. Søgaard

doi:10.33594/000000715

Single-Cell Analysis Reveals That CD47 mRNA Expression Correlates with Immune Cell Activation, Antiviral Isgs, and Cytotoxicity

Lamin B. Cham, Miriam Rosas-Umbert, Lin Lin, Martin Tolstrup, Ole S. Søgaard

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

Abstract

BACKGROUND/AIMS: Immune cells are reported to upregulate CD47 during infection, however, the role of CD47 in innate and adaptive immune cells remains unclear. METHODS: To bridge this knowledge gap, we analysed our single cell (sc)-RNA dataset along with other publicly available sc-RNA datasets from healthy controls, people with HIV-1 (PWH) and COVID-19 patients. We characterized each immune cell based on low, intermediate, and high expression of CD47 . RESULTS: Our analyses revealed that CD47high pDCs and monocytes exhibited relatively higher expression of IFN-α regulatory genes, antiviral interferon-stimulated genes (ISGs) and MHC-I associated genes compared to CD47inter. and CD47low cells. Furthermore, CD47high NK and CD8+ T cells showed higher expression of antiviral ISGs, as well as genes encoding for cytotoxic markers like granzyme B, perforin, granulysin, interferon gamma and NKG7. Additionally, CD47high CD8+ T cells expressed higher levels of PD-1 and LAG-3 genes. Lastly, we found that CD47high B cells had enriched expression of genes involved in cell activation and humoral responses. CONCLUSION: Overall, our analyses revealed that innate and adaptive immune cells expressing elevated activation and functional gene signatures also express higher CD47 levels.

Original language	English
Journal	Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology
Volume	58
Issue	4
Pages (from-to)	322-335
Number of pages	14
ISSN	1015-8987
DOIs	https://doi.org/10.33594/000000715
Publication status	Published - 26 Jul 2024

Keywords

CD47 ; Immune cells ; Transcriptomic ; Infection ; Antiviral ; Cytotoxic
Infection
Antiviral
Cd47
Cytotoxic
Immune cells
Transcriptomic

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Cham, L. B., Rosas-Umbert, M., Lin, L., Tolstrup, M., & Søgaard, O. S. (2024). Single-Cell Analysis Reveals That CD47 mRNA Expression Correlates with Immune Cell Activation, Antiviral Isgs, and Cytotoxicity. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 58(4), 322-335. https://doi.org/10.33594/000000715

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title = "Single-Cell Analysis Reveals That CD47 mRNA Expression Correlates with Immune Cell Activation, Antiviral Isgs, and Cytotoxicity",

abstract = "BACKGROUND/AIMS: Immune cells are reported to upregulate CD47 during infection, however, the role of CD47 in innate and adaptive immune cells remains unclear. METHODS: To bridge this knowledge gap, we analysed our single cell (sc)-RNA dataset along with other publicly available sc-RNA datasets from healthy controls, people with HIV-1 (PWH) and COVID-19 patients. We characterized each immune cell based on low, intermediate, and high expression of CD47 . RESULTS: Our analyses revealed that CD47high pDCs and monocytes exhibited relatively higher expression of IFN-α regulatory genes, antiviral interferon-stimulated genes (ISGs) and MHC-I associated genes compared to CD47inter. and CD47low cells. Furthermore, CD47high NK and CD8+ T cells showed higher expression of antiviral ISGs, as well as genes encoding for cytotoxic markers like granzyme B, perforin, granulysin, interferon gamma and NKG7. Additionally, CD47high CD8+ T cells expressed higher levels of PD-1 and LAG-3 genes. Lastly, we found that CD47high B cells had enriched expression of genes involved in cell activation and humoral responses. CONCLUSION: Overall, our analyses revealed that innate and adaptive immune cells expressing elevated activation and functional gene signatures also express higher CD47 levels.",

keywords = "CD47 ; Immune cells ; Transcriptomic ; Infection ; Antiviral ; Cytotoxic, Infection, Antiviral, Cd47, Cytotoxic, Immune cells, Transcriptomic",

author = "Cham, {Lamin B.} and Miriam Rosas-Umbert and Lin Lin and Martin Tolstrup and S{\o}gaard, {Ole S.}",

note = "Publisher Copyright: {\textcopyright} Copyright by the Author(s). Published by Cell Physiol Biochem Press.",

year = "2024",

month = jul,

day = "26",

doi = "10.33594/000000715",

language = "English",

volume = "58",

pages = "322--335",

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Cham, LB , Rosas-Umbert, M, Lin, L, Tolstrup, M & Søgaard, OS 2024, 'Single-Cell Analysis Reveals That CD47 mRNA Expression Correlates with Immune Cell Activation, Antiviral Isgs, and Cytotoxicity', Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, vol. 58, no. 4, pp. 322-335. https://doi.org/10.33594/000000715

Single-Cell Analysis Reveals That CD47 mRNA Expression Correlates with Immune Cell Activation, Antiviral Isgs, and Cytotoxicity. / Cham, Lamin B.; Rosas-Umbert, Miriam; Lin, Lin et al.
In: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 58, No. 4, 26.07.2024, p. 322-335.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

TY - JOUR

T1 - Single-Cell Analysis Reveals That CD47 mRNA Expression Correlates with Immune Cell Activation, Antiviral Isgs, and Cytotoxicity

AU - Cham, Lamin B.

AU - Rosas-Umbert, Miriam

AU - Lin, Lin

AU - Tolstrup, Martin

AU - Søgaard, Ole S.

N1 - Publisher Copyright: © Copyright by the Author(s). Published by Cell Physiol Biochem Press.

PY - 2024/7/26

Y1 - 2024/7/26

N2 - BACKGROUND/AIMS: Immune cells are reported to upregulate CD47 during infection, however, the role of CD47 in innate and adaptive immune cells remains unclear. METHODS: To bridge this knowledge gap, we analysed our single cell (sc)-RNA dataset along with other publicly available sc-RNA datasets from healthy controls, people with HIV-1 (PWH) and COVID-19 patients. We characterized each immune cell based on low, intermediate, and high expression of CD47 . RESULTS: Our analyses revealed that CD47high pDCs and monocytes exhibited relatively higher expression of IFN-α regulatory genes, antiviral interferon-stimulated genes (ISGs) and MHC-I associated genes compared to CD47inter. and CD47low cells. Furthermore, CD47high NK and CD8+ T cells showed higher expression of antiviral ISGs, as well as genes encoding for cytotoxic markers like granzyme B, perforin, granulysin, interferon gamma and NKG7. Additionally, CD47high CD8+ T cells expressed higher levels of PD-1 and LAG-3 genes. Lastly, we found that CD47high B cells had enriched expression of genes involved in cell activation and humoral responses. CONCLUSION: Overall, our analyses revealed that innate and adaptive immune cells expressing elevated activation and functional gene signatures also express higher CD47 levels.

AB - BACKGROUND/AIMS: Immune cells are reported to upregulate CD47 during infection, however, the role of CD47 in innate and adaptive immune cells remains unclear. METHODS: To bridge this knowledge gap, we analysed our single cell (sc)-RNA dataset along with other publicly available sc-RNA datasets from healthy controls, people with HIV-1 (PWH) and COVID-19 patients. We characterized each immune cell based on low, intermediate, and high expression of CD47 . RESULTS: Our analyses revealed that CD47high pDCs and monocytes exhibited relatively higher expression of IFN-α regulatory genes, antiviral interferon-stimulated genes (ISGs) and MHC-I associated genes compared to CD47inter. and CD47low cells. Furthermore, CD47high NK and CD8+ T cells showed higher expression of antiviral ISGs, as well as genes encoding for cytotoxic markers like granzyme B, perforin, granulysin, interferon gamma and NKG7. Additionally, CD47high CD8+ T cells expressed higher levels of PD-1 and LAG-3 genes. Lastly, we found that CD47high B cells had enriched expression of genes involved in cell activation and humoral responses. CONCLUSION: Overall, our analyses revealed that innate and adaptive immune cells expressing elevated activation and functional gene signatures also express higher CD47 levels.

KW - CD47 ; Immune cells ; Transcriptomic ; Infection ; Antiviral ; Cytotoxic

KW - Infection

KW - Antiviral

KW - Cd47

KW - Cytotoxic

KW - Immune cells

KW - Transcriptomic

UR - http://www.scopus.com/inward/record.url?scp=85200102854&partnerID=8YFLogxK

U2 - 10.33594/000000715

DO - 10.33594/000000715

M3 - Journal article

C2 - 39074350

AN - SCOPUS:85200102854

SN - 1015-8987

VL - 58

SP - 322

EP - 335

JO - Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

JF - Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

IS - 4

ER -

Single-Cell Analysis Reveals That CD47 mRNA Expression Correlates with Immune Cell Activation, Antiviral Isgs, and Cytotoxicity

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