Silencing of endogenous envelope genes in human choriocarcinoma cells shows that envPb1 is involved in heterotypic cell fusions

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Silencing of endogenous envelope genes in human choriocarcinoma cells shows that envPb1 is involved in heterotypic cell fusions. / Aagaard, Lars; Bjerregaard, Bolette; Kjeldbjerg, Anders L; Pedersen, Finn Skou; Larsson, Lars-Inge; Rossi, John J.

In: Journal of General Virology, Vol. 93, No. 8, 2012, p. 1696-1699.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

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Aagaard, L, Bjerregaard, B, Kjeldbjerg, AL, Pedersen, FS, Larsson, L-I & Rossi, JJ 2012, 'Silencing of endogenous envelope genes in human choriocarcinoma cells shows that envPb1 is involved in heterotypic cell fusions', Journal of General Virology, vol. 93, no. 8, pp. 1696-1699. https://doi.org/10.1099/vir.0.041764-0

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Author

Aagaard, Lars ; Bjerregaard, Bolette ; Kjeldbjerg, Anders L ; Pedersen, Finn Skou ; Larsson, Lars-Inge ; Rossi, John J. / Silencing of endogenous envelope genes in human choriocarcinoma cells shows that envPb1 is involved in heterotypic cell fusions. In: Journal of General Virology. 2012 ; Vol. 93, No. 8. pp. 1696-1699.

Bibtex

@article{a6aeee01f8854a129928391f4dc72124,
title = "Silencing of endogenous envelope genes in human choriocarcinoma cells shows that envPb1 is involved in heterotypic cell fusions",
abstract = "Syncytin-1 and envPb1 are two conserved envelope genes in the human genome encoded by single loci from the HERV-W and -Pb families, respectively. To characterize the role of these envelope proteins in cell-cell fusion, we have developed lentiviral vectors expressing short hairpin RNAs for stable knockdown of syncytin-1 and envPb1. Analysis of heterotypic fusion activity between trophoblast-derived choriocarcinoma BeWo cells, in which syncytin-1 and envPb1 are specifically silenced, and endothelial cells demonstrate that both syncytin-1 and envPb1 are important to fusion. The ability to fuse cells makes syncytin-1 and envPb1 attractive candidate molecules in therapy against cancer. Our available vectors may help to eventually decipher roles for these genes in human health and/or disease.",
author = "Lars Aagaard and Bolette Bjerregaard and Kjeldbjerg, {Anders L} and Pedersen, {Finn Skou} and Lars-Inge Larsson and Rossi, {John J}",
year = "2012",
doi = "10.1099/vir.0.041764-0",
language = "English",
volume = "93",
pages = "1696--1699",
journal = "Journal of General Virology",
issn = "0022-1317",
publisher = "Society for General Microbiology",
number = "8",

}

RIS

TY - JOUR

T1 - Silencing of endogenous envelope genes in human choriocarcinoma cells shows that envPb1 is involved in heterotypic cell fusions

AU - Aagaard, Lars

AU - Bjerregaard, Bolette

AU - Kjeldbjerg, Anders L

AU - Pedersen, Finn Skou

AU - Larsson, Lars-Inge

AU - Rossi, John J

PY - 2012

Y1 - 2012

N2 - Syncytin-1 and envPb1 are two conserved envelope genes in the human genome encoded by single loci from the HERV-W and -Pb families, respectively. To characterize the role of these envelope proteins in cell-cell fusion, we have developed lentiviral vectors expressing short hairpin RNAs for stable knockdown of syncytin-1 and envPb1. Analysis of heterotypic fusion activity between trophoblast-derived choriocarcinoma BeWo cells, in which syncytin-1 and envPb1 are specifically silenced, and endothelial cells demonstrate that both syncytin-1 and envPb1 are important to fusion. The ability to fuse cells makes syncytin-1 and envPb1 attractive candidate molecules in therapy against cancer. Our available vectors may help to eventually decipher roles for these genes in human health and/or disease.

AB - Syncytin-1 and envPb1 are two conserved envelope genes in the human genome encoded by single loci from the HERV-W and -Pb families, respectively. To characterize the role of these envelope proteins in cell-cell fusion, we have developed lentiviral vectors expressing short hairpin RNAs for stable knockdown of syncytin-1 and envPb1. Analysis of heterotypic fusion activity between trophoblast-derived choriocarcinoma BeWo cells, in which syncytin-1 and envPb1 are specifically silenced, and endothelial cells demonstrate that both syncytin-1 and envPb1 are important to fusion. The ability to fuse cells makes syncytin-1 and envPb1 attractive candidate molecules in therapy against cancer. Our available vectors may help to eventually decipher roles for these genes in human health and/or disease.

U2 - 10.1099/vir.0.041764-0

DO - 10.1099/vir.0.041764-0

M3 - Journal article

C2 - 22573740

VL - 93

SP - 1696

EP - 1699

JO - Journal of General Virology

JF - Journal of General Virology

SN - 0022-1317

IS - 8

ER -