Serum induced degradation of 3D DNA box origami observed by high speed atomic force microscope

Zaixing Jiang, Shuai Zhang, Chuanxu Yang, Jørgen Kjems, Yudong Huang, Flemming Besenbacher, MD Dong

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review


3D DNA origami holds tremendous potential to encapsulate and selectively release therapeutic drugs. Observations of real-time performance of 3D DNA origami structures in physiological environment will contribute much to its further applications. Here, we investigate the degradation kinetics of 3D DNA box origami in serum using high-speed atomic force microscope optimized for
imaging 3D DNA origami in real time. The time resolution allows
characterizing the stages of serum effects on individual 3D DNA box origami with nanometer resolution. Our results indicate that the whole digest process is a combination of a rapid collapse phase and a slow degradation phase. The damages of box origami mainly happen in the collapse phase. Thus, the structure stability of 3D DNA box origami should be further improved, especially in the collapse phase, before clinical applications
Original languageEnglish
JournalNano Research
Pages (from-to)2170-2178
Number of pages9
Publication statusPublished - Jul 2015


  • 3D DNA box origami, high-speed AFM, stability, serum, kinetic


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