Septin9 is involved in septin filament formation and cellular stability.

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Septin9 is involved in septin filament formation and cellular stability. / Füchtbauer, Annette C.; Lassen, Louise Berkhoudt; Jensen, Astrid Bøgh; Howard, Jennifer ; Quiroga, Adan De Salas; Warming, Søren; Sørensen, Annette Balle; Pedersen, Finn Skou; Füchtbauer, Ernst-Martin.

In: Biological Chemistry, Vol. 392, No. 8-9, 08.2011, p. 769-777.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Füchtbauer, AC, Lassen, LB, Jensen, AB, Howard, J, Quiroga, ADS, Warming, S, Sørensen, AB, Pedersen, FS & Füchtbauer, E-M 2011, 'Septin9 is involved in septin filament formation and cellular stability.' Biological Chemistry, vol. 392, no. 8-9, pp. 769-777.

APA

Füchtbauer, A. C., Lassen, L. B., Jensen, A. B., Howard, J., Quiroga, A. D. S., Warming, S., ... Füchtbauer, E-M. (2011). Septin9 is involved in septin filament formation and cellular stability. Biological Chemistry, 392(8-9), 769-777.

CBE

MLA

Vancouver

Füchtbauer AC, Lassen LB, Jensen AB, Howard J, Quiroga ADS, Warming S et al. Septin9 is involved in septin filament formation and cellular stability. Biological Chemistry. 2011 Aug;392(8-9):769-777.

Author

Füchtbauer, Annette C. ; Lassen, Louise Berkhoudt ; Jensen, Astrid Bøgh ; Howard, Jennifer ; Quiroga, Adan De Salas ; Warming, Søren ; Sørensen, Annette Balle ; Pedersen, Finn Skou ; Füchtbauer, Ernst-Martin. / Septin9 is involved in septin filament formation and cellular stability. In: Biological Chemistry. 2011 ; Vol. 392, No. 8-9. pp. 769-777.

Bibtex

@article{3b1adcba2e2a4e68a374fe3f606506cd,
title = "Septin9 is involved in septin filament formation and cellular stability.",
abstract = "Septin9 (Sept9) is a member of the filament-forming septin family of structural proteins and is associated with a variety of cancers and with hereditary neuralgic amyotrophy. We have generated mice with constitutive and conditional Sept9 knockout alleles. Homozygous deletion of Sept9 results in embryonic lethality around day 10 of gestation whereas mice homozygous for the conditional allele develop normally. Here we report the consequences of homozygous loss of Sept9 in immortalized murine embryonic fibroblasts. Proliferation rate was not changed but cells without Sept9 had an altered morphology compared to normal cells, particularly under low serum stress. Abnormal, fragmented, and multiple nuclei were more frequent in cells without Sept9. Cell migration, as measured by gap-filling and filter-invasion assays, was impaired, but individual cells did not move less than wild-type cells. Sept9 knockout cells showed a reduced resistance to hypo-osmotic stress. Stress fiber and vinculin staining at focal adhesion points was less prominent. Long septin filaments stained for Sept7 disappeared. Instead, staining was found in short, often curved filaments and rings. Furthermore, Sept7 was no longer localized to the mitotic spindle. Together, these data reveal the importance of Sept9 for septin filament formation and general cell stability.",
author = "F{\"u}chtbauer, {Annette C.} and Lassen, {Louise Berkhoudt} and Jensen, {Astrid B{\o}gh} and Jennifer Howard and Quiroga, {Adan De Salas} and S{\o}ren Warming and S{\o}rensen, {Annette Balle} and Pedersen, {Finn Skou} and Ernst-Martin F{\"u}chtbauer",
year = "2011",
month = "8",
language = "English",
volume = "392",
pages = "769--777",
journal = "Biological Chemistry",
issn = "1431-6730",
publisher = "Walterde Gruyter GmbH",
number = "8-9",

}

RIS

TY - JOUR

T1 - Septin9 is involved in septin filament formation and cellular stability.

AU - Füchtbauer, Annette C.

AU - Lassen, Louise Berkhoudt

AU - Jensen, Astrid Bøgh

AU - Howard, Jennifer

AU - Quiroga, Adan De Salas

AU - Warming, Søren

AU - Sørensen, Annette Balle

AU - Pedersen, Finn Skou

AU - Füchtbauer, Ernst-Martin

PY - 2011/8

Y1 - 2011/8

N2 - Septin9 (Sept9) is a member of the filament-forming septin family of structural proteins and is associated with a variety of cancers and with hereditary neuralgic amyotrophy. We have generated mice with constitutive and conditional Sept9 knockout alleles. Homozygous deletion of Sept9 results in embryonic lethality around day 10 of gestation whereas mice homozygous for the conditional allele develop normally. Here we report the consequences of homozygous loss of Sept9 in immortalized murine embryonic fibroblasts. Proliferation rate was not changed but cells without Sept9 had an altered morphology compared to normal cells, particularly under low serum stress. Abnormal, fragmented, and multiple nuclei were more frequent in cells without Sept9. Cell migration, as measured by gap-filling and filter-invasion assays, was impaired, but individual cells did not move less than wild-type cells. Sept9 knockout cells showed a reduced resistance to hypo-osmotic stress. Stress fiber and vinculin staining at focal adhesion points was less prominent. Long septin filaments stained for Sept7 disappeared. Instead, staining was found in short, often curved filaments and rings. Furthermore, Sept7 was no longer localized to the mitotic spindle. Together, these data reveal the importance of Sept9 for septin filament formation and general cell stability.

AB - Septin9 (Sept9) is a member of the filament-forming septin family of structural proteins and is associated with a variety of cancers and with hereditary neuralgic amyotrophy. We have generated mice with constitutive and conditional Sept9 knockout alleles. Homozygous deletion of Sept9 results in embryonic lethality around day 10 of gestation whereas mice homozygous for the conditional allele develop normally. Here we report the consequences of homozygous loss of Sept9 in immortalized murine embryonic fibroblasts. Proliferation rate was not changed but cells without Sept9 had an altered morphology compared to normal cells, particularly under low serum stress. Abnormal, fragmented, and multiple nuclei were more frequent in cells without Sept9. Cell migration, as measured by gap-filling and filter-invasion assays, was impaired, but individual cells did not move less than wild-type cells. Sept9 knockout cells showed a reduced resistance to hypo-osmotic stress. Stress fiber and vinculin staining at focal adhesion points was less prominent. Long septin filaments stained for Sept7 disappeared. Instead, staining was found in short, often curved filaments and rings. Furthermore, Sept7 was no longer localized to the mitotic spindle. Together, these data reveal the importance of Sept9 for septin filament formation and general cell stability.

M3 - Journal article

VL - 392

SP - 769

EP - 777

JO - Biological Chemistry

JF - Biological Chemistry

SN - 1431-6730

IS - 8-9

ER -