Second harmonic generation microscopy investigation of the crystalline ultrastructure of three barley starch lines affected by hydration

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DOI

  • Richard Cisek, Department of Chemical and Physical Sciences, Department of Physics and Institute for Optical Sciences, University of Toronto, Canada
  • Danielle Tokarz, Department of Chemical and Physical Sciences, Department of Physics and Institute for Optical Sciences, University of Toronto, Canada
  • Martin Steup, Department of Molecular and Cellular Biology, College of Biological Science, Summerlee Science Complex, University of Guelph, Canada
  • Ian J Tetlow, Department of Molecular and Cellular Biology, College of Biological Science, Summerlee Science Complex, University of Guelph, Canada
  • Michael J Emes, Department of Molecular and Cellular Biology, College of Biological Science, Summerlee Science Complex, University of Guelph, Canada
  • Kim Hebelstrup
  • Andreas Blennow, Department of Plant and Environmental Sciences, University of Copenhagen, Denmark
  • Virginijus Barzda, Department of Chemical and Physical Sciences, Department of Physics and Institute for Optical Sciences, University of Toronto, Canada
Second harmonic generation (SHG) microscopy is employed to study changes in crystalline organization due to altered gene expression and hydration in barley starch granules. SHG intensity and susceptibility ratio values (R’SHG) are obtained using reduced Stokes-Mueller polarimetric microscopy. The maximum R’SHG values occur at moderate moisture indicating the narrowest orientation distribution of nonlinear dipoles from the cylindrical axis of glucan helices. The maximum SHG intensity occurs at the highest moisture and amylopectin content. These results support the hypothesis that SHG is caused by ordered hydrogen and hydroxyl bond networks which increase with hydration of starch granules.
Original languageEnglish
JournalBiomedical Optics Express
Volume6
Issue10
Pages (from-to)3694-3700
Number of pages7
ISSN2156-7085
DOIs
Publication statusPublished - 1 Sep 2015

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