Saturated or unsaturated fat supplemented maternal diets influence omental adipose tissue proteome of suckling goat-kids

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

  • Laura Restelli, Universita degli Studi di Milano
  • ,
  • Andreia T. Marques, Universita degli Studi di Milano
  • ,
  • Giovanni Savoini, Universita degli Studi di Milano
  • ,
  • Guido Invernizzi, Universita degli Studi di Milano
  • ,
  • Michela Carisetti, Universita degli Studi di Milano
  • ,
  • Cristina Lecchi, Universita degli Studi di Milano
  • ,
  • Emoke Bendixen
  • Fabrizio Ceciliani, Universita degli Studi di Milano

The aim of the present study was to investigate how maternal diet can influence the adipose tissue of goat kids. Omental adipose tissue proteomes of goat-kids from mothers fed with diet enriched with stearic acid (ST-kids), fish oil (FO-kids) and standard diets (CTRL) were determined by quantitative iTRAQ 2D-LC-MS/MS analysis. Twenty proteins were found to be differentially expressed in suckling kids' omental adipose tissue. Stearic acid induces changes in a higher number of proteins when compared to fish oil. Eleven proteins, namely AARS, ECl1, PMSC2, CP, HSPA8, GPD1, RPL7, OGDH, RPL24, FGA and RPL5 were decreased in ST-kids only. Four proteins, namely DLST, EEF1G, BCAP31 and RALA were decreased in FO-kids only, and one, NUCKS1, was increased. Four proteins, namely PMSC1, PPIB, TUB5 x 2 and EIF5A1, were be less abundant in both ST- and FO- kids. Most of the protein whose abundance was decreased in ST kids (10 out of 15) are involved in protein metabolism and catabolism pathways. Qualitative gene expression analysis confirmed that all the proteins identified by mass spectrometry, with the exception of FGA, were produced by adipose tissue. Quantitative gene expression analysis demonstrated that two proteins, namely CP, a minor acute phase protein, and ECl1, involved in fatty acid beta oxidation, were downregulated at mRNA level as well. ECl1 gene expression was downregulated in ST-kids AT as compared to Ctrl-kids and CP was downregulated in both ST- and FO-kids. The present results demonstrate that it is possible to influence adipose goat-kid proteome by modifying the maternal diet.

Original languageEnglish
JournalResearch in Veterinary Science
Volume125
Pages (from-to)451-458
Number of pages8
ISSN0034-5288
DOIs
Publication statusPublished - Aug 2019

    Research areas

  • Adipose tissue, Fish oil, Goat, Peripartum, Proteomics, Stearic acid, OXIDATION, MESSENGER-RNA ABUNDANCE, ACID SECRETION, LIPID-METABOLISM, IMMUNE FUNCTIONS, MILK-FAT, GENE-EXPRESSION, LINSEED OIL, FISH-OIL, SUNFLOWER-SEED OIL

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