Sandarakinorhabdus cyanobacteriorum sp. nov., a novel bacterium isolated from cyanobacterial aggregates in a eutrophic lake

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DOI

  • Haiyuan Cai, 1​State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing, PR China.
  • ,
  • Henglin Cui, 2​School of Food & Biological Engineering, Jiangsu University, Zhenjiang, PR China.
  • ,
  • Yonghui Zeng
  • Mingli An, 5​Key Laboratory of Microbial Engineering at the Institute of Biology, Henan Academy of Sciences, Zhengzhou, PR China.
  • ,
  • Helong Jiang, 1​State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing, PR China.

A bacterial strain, designated TH057T, was isolated from cyanobacterial aggregates in a eutrophic lake in China. Cells were observed to be slightly curved, rod-shaped, capsule-forming and stained Gram-negative. Optimal growth was obtained at pH 7.0 (range: pH 5-9) and 30 °C (range: 20-37 °C) in R2A broth. According to the absorption spectrum, carotenoids (455 and 490 nm) and light-harvesting complex LHI (857 nm) were present in the cells. The cells were found to be positive for oxidase and catalase activities. The major respiratory quinone was ubiquinone Q-10. The major fatty acids were identified as C17 : 1ω6c, C16 : 1ω7c/C16 : 1ω6c, C18 : 1ω6c/C18 : 1ω7c and C16 : 0. The major polar lipids were found to consist of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, unidentified glycolipid and two sphingoglycolipids. Strain TH057Tshared highest 16S rRNA gene sequence similarity to Sandarakinorhabdus limnophila so42T(96.8 %), followed by Polymorphobacter fuscus D40PT(95.8 %). The genomic G+C content of strain TH057Twas 66.1 mol% based on total genome calculations. The average nucleotide identity and the digital DNA-DNA hybridization value for the complete genomes were 81.0 and 23.0 % between strain TH057Tand Sandarakinorhabdus limnophila so42T. The phenotypic, chemotaxonomic and phylogenetic properties, and genome analysis suggested that strain TH057Trepresents a novel species within the genus Sandarakinorhabdus, for which the name Sandarakinorhabduscyanobacteriorum sp. nov. is proposed. The type strain is TH057T(=CGMCC 1.15803T=LMG 30294T).

Original languageEnglish
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume68
Issue3
Pages (from-to)730-735
Number of pages6
ISSN1466-5026
DOIs
Publication statusPublished - 2018

    Research areas

  • cyanobacterial aggregates, Sandarakinorhabdus cyanobacteriorum sp. nov., Nucleic Acid Hybridization, RNA, Ribosomal, 16S/genetics, Phospholipids/chemistry, Phylogeny, Sequence Analysis, DNA, Ubiquinone/chemistry, Sphingomonadaceae/classification, Lakes/microbiology, Base Composition, Bacterial Typing Techniques, China, Cyanobacteria, Fatty Acids/chemistry, DNA, Bacterial/genetics

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