Salivary proteotypes of gingivitis tolerance and resilience

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  • Nagihan Bostanci, Karolinska Institutet, Stockholm, Sweden, Sweden
  • Angelika Silbereisen, Karolinska Institutet, Stockholm, Sweden, Sweden
  • Kai Bao, Karolinska Institutet, Stockholm, Sweden, Sweden
  • Jonas Grossmann, University of Zurich, Switzerland, Switzerland
  • Paolo Nanni, University of Zurich, Switzerland, Switzerland
  • Claudia Fernandez, University of Zurich, Switzerland, Switzerland
  • Gustavo G Nascimento
  • Georgios N Belibasakis, Karolinska Institutet, Stockholm, Sweden, Sweden
  • Rodrigo López

AIM: This study aimed to characterize the salivary proteome during the induction and resolution of gingival inflammation in the course of human experimental gingivitis (EG), and to cluster the proteomic profiles based on the clinically-defined 'slow' and 'fast' response patterns.

MATERIALS AND METHODS: A total of 50 unstimulated whole saliva were obtained from the EG model which was induced over 21 days (day 0, 7, 14, 21), followed by a two-week resolution phase (day 35). Label-free quantitative proteomics using liquid chromatography-tandem mass spectrometry was applied. Regulated proteins were subject to gene ontology enrichment analysis.

RESULTS: A total of 804 human proteins were quantified by ≥2 peptides. Principal component analysis depicted significant differences between 'fast' and 'slow' responders. Despite gingival and plaque scores being similar at baseline among the two groups, 'fast' responders presented with 48 proteins that were at >4-fold higher levels than 'slow' responders. These up-regulated proteins showed enrichment in "antigen presentation" and "proteolysis".

CONCLUSIONS: Together, these findings highlight the utility of integrative systems-level quantitative proteomic approaches to unravel the molecular basis of 'salivary proteotypes' associated with gingivitis dubbed as 'fast' and 'slow' responders. Hence, these differential responses may help prognosticate individual susceptibility to gingival inflammation.

Original languageEnglish
JournalJournal of Clinical Periodontology
Pages (from-to)1304-1316
Number of pages13
Publication statusPublished - Nov 2020

Bibliographical note

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    Research areas

  • biomarkers, experimental gingivitis, proteomics, saliva, salivary proteotypes

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