RNAi using a chitosan/siRNA nanoparticle system: in vitro and in vivo applications

Research output: Contribution to book/anthology/report/proceedingBook chapterResearchpeer-review

Delivery is a key issue in development of clinically relevant RNAi therapeutics. Polymeric nanoparticles formed by self-assembly of polycations with siRNA can be used for extracellular delivery, cellular uptake and intracellular trafficking as a strategy to improve the therapeutic potential of siRNA. This chapter describes a chitosan-based nanoparticle system for in vitro and in vivo transfection of siRNA into cells. The method exploits the mucoadhesive and mucopermeable properties of this cationic polysaccharide to deliver siRNA across mucosal epithelium and provides a platform for targeting human diseases with RNAi therapeutics.
Original languageEnglish
Title of host publicationMethods in Molecular Biology
Number of pages10
Volume555
Publication year2009
Pages77-86
Chapter6
DOIs
Publication statusPublished - 2009
SeriesMethods in Molecular Biology
ISSN1064-3745

    Research areas

  • Administration, Intranasal, Animals, Cells, Cultured, Chitosan, Drug Carriers, Humans, Lung, Macrophages, Mice, Nanoparticles, Peritoneum, RNA Interference, RNA, Small Interfering, Transfection, Tumor Necrosis Factor-alpha

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