RNA is a key component of extracellular DNA networks in Pseudomonas aeruginosa biofilms

Sudarsan Mugunthan, Lan Li Wong, Fernaldo Richtia Winnerdy, Stephen Summers, Muhammad Hafiz Bin Ismail, Yong Hwee Foo, Tavleen Kaur Jaggi, Oliver W. Meldrum, Pei Yee Tiew, Sanjay H. Chotirmall, Scott A. Rice, Anh Tuân Phan, Staffan Kjelleberg*, Thomas Seviour*

*Corresponding author for this work

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Abstract

The extracellular matrix of bacterial biofilms consists of diverse components including polysaccharides, proteins and DNA. Extracellular RNA (eRNA) can also be present, contributing to the structural integrity of biofilms. However, technical difficulties related to the low stability of RNA make it difficult to understand the precise roles of eRNA in biofilms. Here, we show that eRNA associates with extracellular DNA (eDNA) to form matrix fibres in Pseudomonas aeruginosa biofilms, and the eRNA is enriched in certain bacterial RNA transcripts. Degradation of eRNA associated with eDNA led to a loss of eDNA fibres and biofilm viscoelasticity. Compared with planktonic and biofilm cells, the biofilm matrix was enriched in specific mRNA transcripts, including lasB (encoding elastase). The mRNA transcripts colocalised with eDNA fibres in the biofilm matrix, as shown by single molecule inexpensive FISH microscopy (smiFISH). The lasB mRNA was also observed in eDNA fibres in a clinical sputum sample positive for P. aeruginosa. Thus, our results indicate that the interaction of specific mRNAs with eDNA facilitates the formation of viscoelastic networks in the matrix of Pseudomonas aeruginosa biofilms.

Original languageEnglish
Article number7772
JournalNature Communications
Volume14
Issue1
ISSN2041-1723
DOIs
Publication statusPublished - Nov 2023

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