Requirements for chromatin reassembly during transcriptional downregulation of a heat shock gene in Saccharomyces cerevisiae

TY - JOUR

T1 - Requirements for chromatin reassembly during transcriptional downregulation of a heat shock gene in Saccharomyces cerevisiae

AU - Jensen, Mette M

AU - Christensen, Marianne Skovgaard

AU - Bonven, Bjarne

AU - Jensen, Torben H

PY - 2008/6/1

Y1 - 2008/6/1

N2 - Heat shock genes respond to moderate heat stress by a wave of transcription. The induction phase is accompanied by the massive eviction of histones, which later reassemble with DNA during the ensuing phase of transcription downregulation. In this article, we identify determinants of this reassembly throughout the heat shock protein 104 gene (HSP104) transcription unit. The results show that, although histone H3 lacking amino acids 4-30 of its N-terminal tail (H3Delta4-30) is normally deposited, reassembly of H3Delta4-40 is obliterated with an accompanying sustained transcription. On mutation of the histone chaperones Spt6p and Spt16p, but not Asf1p, reassociation of H3 with DNA is compromised. However, despite a lasting open chromatin structure, transcription ceases normally in the spt6 mutant. Thus, transcriptional downregulation can be uncoupled from histone redeposition and ongoing transcription is not required to prevent chromatin reassembly.

AB - Heat shock genes respond to moderate heat stress by a wave of transcription. The induction phase is accompanied by the massive eviction of histones, which later reassemble with DNA during the ensuing phase of transcription downregulation. In this article, we identify determinants of this reassembly throughout the heat shock protein 104 gene (HSP104) transcription unit. The results show that, although histone H3 lacking amino acids 4-30 of its N-terminal tail (H3Delta4-30) is normally deposited, reassembly of H3Delta4-40 is obliterated with an accompanying sustained transcription. On mutation of the histone chaperones Spt6p and Spt16p, but not Asf1p, reassociation of H3 with DNA is compromised. However, despite a lasting open chromatin structure, transcription ceases normally in the spt6 mutant. Thus, transcriptional downregulation can be uncoupled from histone redeposition and ongoing transcription is not required to prevent chromatin reassembly.

KW - Chromatin

KW - DNA

KW - Gene Deletion

KW - Gene Expression Regulation

KW - Gene Expression Regulation, Fungal

KW - Genotype

KW - Heat-Shock Proteins

KW - Histones

KW - In Situ Hybridization, Fluorescence

KW - Mutation

KW - Protein Structure, Tertiary

KW - Saccharomyces cerevisiae

KW - Saccharomyces cerevisiae Proteins

KW - Time Factors

KW - Transcription, Genetic

U2 - 10.1111/j.1742-4658.2008.06451.x

DO - 10.1111/j.1742-4658.2008.06451.x

M3 - Journal article

C2 - 18445041

SN - 1742-464X

VL - 275

SP - 2956

EP - 2964

JO - FEBS journal

JF - FEBS journal

IS - 11

ER -