Requirements for chromatin reassembly during transcriptional downregulation of a heat shock gene in S. cerevisiae

Mette Moesgaard Jensen; Marianne Skovgaard Christensen; Bjarne Juul Bonven; Torben Heick Jensen

Requirements for chromatin reassembly during transcriptional downregulation of a heat shock gene in S. cerevisiae

Mette Moesgaard Jensen, Marianne Skovgaard Christensen, Bjarne Juul Bonven, Torben Heick Jensen

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

Abstract

Heat shock genes respond to moderate heat stress by a wave of transcription. The induction phase is accompanied by massive eviction of histones, which later reassemble with DNA during the ensuing phase of transcription downregulation. Here, we identify determinants of this reassembly throughout the HSP104 transcription unit. The results show that while histone H3 lacking amino acids 4 through 30 of its N-terminal tail (H3Δ4-30) is normally deposited, reassembly of H3Δ4-40 is obliterated with an accompanying sustained transcription. Upon mutation of histone chaperones Spt6p and Spt16p, but not Asf1p, reassociation of H3 with DNA is compromised. However, despite a lasting open chromatin structure, transcription ceases normally in the spt6 mutant. Thus, transcriptional downregulation can be uncoupled from histone redepositioning and ongoing transcription is not required to prevent chromatin reassembly.

Original language	English
Journal	FEBS journal
Volume	275
Issue	11
Pages (from-to)	2956-2964
Number of pages	9
ISSN	1742-464X
Publication status	Published - 2008

Keywords

Histone eviction
Histone reassembly
Histone chaperones
HSP104
SPT6
SPT16

Cite this

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title = "Requirements for chromatin reassembly during transcriptional downregulation of a heat shock gene in S. cerevisiae",

abstract = "Heat shock genes respond to moderate heat stress by a wave of transcription. The induction phase is accompanied by massive eviction of histones, which later reassemble with DNA during the ensuing phase of transcription downregulation. Here, we identify determinants of this reassembly throughout the HSP104 transcription unit. The results show that while histone H3 lacking amino acids 4 through 30 of its N-terminal tail (H3Δ4-30) is normally deposited, reassembly of H3Δ4-40 is obliterated with an accompanying sustained transcription. Upon mutation of histone chaperones Spt6p and Spt16p, but not Asf1p, reassociation of H3 with DNA is compromised. However, despite a lasting open chromatin structure, transcription ceases normally in the spt6 mutant. Thus, transcriptional downregulation can be uncoupled from histone redepositioning and ongoing transcription is not required to prevent chromatin reassembly.",

keywords = "Histone eviction, Histone reassembly, Histone chaperones, HSP104, SPT6, SPT16",

author = "Jensen, {Mette Moesgaard} and Christensen, {Marianne Skovgaard} and Bonven, {Bjarne Juul} and Jensen, {Torben Heick}",

year = "2008",

language = "English",

volume = "275",

pages = "2956--2964",

journal = " FEBS journal",

issn = "1742-464X",

publisher = "Wiley-Blackwell Publishing Ltd.",

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Requirements for chromatin reassembly during transcriptional downregulation of a heat shock gene in S. cerevisiae. / Jensen, Mette Moesgaard; Christensen, Marianne Skovgaard; Bonven, Bjarne Juul et al.
In: FEBS journal, Vol. 275, No. 11, 2008, p. 2956-2964.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

TY - JOUR

T1 - Requirements for chromatin reassembly during transcriptional downregulation of a heat shock gene in S. cerevisiae

AU - Jensen, Mette Moesgaard

AU - Christensen, Marianne Skovgaard

AU - Bonven, Bjarne Juul

AU - Jensen, Torben Heick

PY - 2008

Y1 - 2008

N2 - Heat shock genes respond to moderate heat stress by a wave of transcription. The induction phase is accompanied by massive eviction of histones, which later reassemble with DNA during the ensuing phase of transcription downregulation. Here, we identify determinants of this reassembly throughout the HSP104 transcription unit. The results show that while histone H3 lacking amino acids 4 through 30 of its N-terminal tail (H3Δ4-30) is normally deposited, reassembly of H3Δ4-40 is obliterated with an accompanying sustained transcription. Upon mutation of histone chaperones Spt6p and Spt16p, but not Asf1p, reassociation of H3 with DNA is compromised. However, despite a lasting open chromatin structure, transcription ceases normally in the spt6 mutant. Thus, transcriptional downregulation can be uncoupled from histone redepositioning and ongoing transcription is not required to prevent chromatin reassembly.

AB - Heat shock genes respond to moderate heat stress by a wave of transcription. The induction phase is accompanied by massive eviction of histones, which later reassemble with DNA during the ensuing phase of transcription downregulation. Here, we identify determinants of this reassembly throughout the HSP104 transcription unit. The results show that while histone H3 lacking amino acids 4 through 30 of its N-terminal tail (H3Δ4-30) is normally deposited, reassembly of H3Δ4-40 is obliterated with an accompanying sustained transcription. Upon mutation of histone chaperones Spt6p and Spt16p, but not Asf1p, reassociation of H3 with DNA is compromised. However, despite a lasting open chromatin structure, transcription ceases normally in the spt6 mutant. Thus, transcriptional downregulation can be uncoupled from histone redepositioning and ongoing transcription is not required to prevent chromatin reassembly.

KW - Histone eviction

KW - Histone reassembly

KW - Histone chaperones

KW - HSP104

KW - SPT6

KW - SPT16

M3 - Journal article

SN - 1742-464X

VL - 275

SP - 2956

EP - 2964

JO - FEBS journal

JF - FEBS journal

IS - 11

ER -

Requirements for chromatin reassembly during transcriptional downregulation of a heat shock gene in S. cerevisiae

Abstract

Keywords

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