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Relationships between human vitality and mitochondrial respiratory parameters, reactive oxygen species production and dNTP levels in peripheral blood mononuclear cells

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  • Scott Maynard, University of Copenhagen, Denmark
  • Guido Keijzers, University of Copenhagen, Denmark
  • Martin Gram, University of Copenhagen, Denmark
  • Claus Desler, University of Copenhagen, Denmark
  • Laila Bendix, Afdeling for Social Medicin, Denmark
  • Esben Budtz-Jørgensen, University of Copenhagen, Denmark
  • Drude Molbo, University of Copenhagen, Denmark
  • Deborah L Croteau
  • ,
  • Merete Osler, University of Southern Denmark, Denmark
  • Tinna Stevnsner
  • Lene Juel Rasmussen, University of Copenhagen, Denmark
  • Flemming Dela, University of Copenhagen, Denmark
  • Kirsten Avlund, University of Copenhagen, Denmark
  • Vilhelm Bohr, Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health, Denmark
Low vitality (a component of fatigue) in middle-aged and older adults is an important complaint often identified as a symptom of a disease state or side effect of a treatment. No studies to date have investigated the potential link between dysfunctional mitochondrial ATP production and low vitality. Therefore, we measured a number of cellular parameters related to mitochondrial activity in peripheral blood mononuclear cells (PBMCs) isolated from middle-aged men, and tested for association with vitality. These parameters estimate mitochondrial respiration, reactive oxygen species (ROS) production, and deoxyribonucleotide (dNTP) balance in PBMCs. The population was drawn from the Metropolit cohort of men born in 1953. Vitality level was estimated from the Medical Outcomes Study Short Form 36 (SF-36) vitality scale. We found that vitality score had no association with any of the mitochondrial respiration parameters. However, vitality score was inversely associated with cellular ROS production and cellular deoxythymidine triphosphate (dTTP) levels and positively associated with deoxycytidine triphosphate (dCTP) levels. We conclude that self-reported persistent low vitality is not associated with specific aspects of mitochondrial oxidative phosphorylation capacity in PBMCs, but may have other underlying cellular dysfunctions that contribute to dNTP imbalance and altered ROS production.
Original languageEnglish
JournalNature Communications
Pages (from-to)850-864
Number of pages15
Publication statusPublished - 11 Nov 2013

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