Reduction of dual-species biofilm after sonic- or ultrasonic-activated irrigation protocols: A laboratory study

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Reduction of dual-species biofilm after sonic- or ultrasonic-activated irrigation protocols : A laboratory study. / Hoedke, Daniela; Kaulika, Namira; Dommisch, Henrik; Schlafer, Sebastian; Shemesh, Hagay; Bitter, Kerstin.

In: International Endodontic Journal, Vol. 54, No. 12, 12.2021, p. 2219-2228.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Hoedke, D, Kaulika, N, Dommisch, H, Schlafer, S, Shemesh, H & Bitter, K 2021, 'Reduction of dual-species biofilm after sonic- or ultrasonic-activated irrigation protocols: A laboratory study', International Endodontic Journal, vol. 54, no. 12, pp. 2219-2228. https://doi.org/10.1111/iej.13618

APA

Hoedke, D., Kaulika, N., Dommisch, H., Schlafer, S., Shemesh, H., & Bitter, K. (2021). Reduction of dual-species biofilm after sonic- or ultrasonic-activated irrigation protocols: A laboratory study. International Endodontic Journal, 54(12), 2219-2228. https://doi.org/10.1111/iej.13618

CBE

Hoedke D, Kaulika N, Dommisch H, Schlafer S, Shemesh H, Bitter K. 2021. Reduction of dual-species biofilm after sonic- or ultrasonic-activated irrigation protocols: A laboratory study. International Endodontic Journal. 54(12):2219-2228. https://doi.org/10.1111/iej.13618

MLA

Vancouver

Hoedke D, Kaulika N, Dommisch H, Schlafer S, Shemesh H, Bitter K. Reduction of dual-species biofilm after sonic- or ultrasonic-activated irrigation protocols: A laboratory study. International Endodontic Journal. 2021 Dec;54(12):2219-2228. https://doi.org/10.1111/iej.13618

Author

Hoedke, Daniela ; Kaulika, Namira ; Dommisch, Henrik ; Schlafer, Sebastian ; Shemesh, Hagay ; Bitter, Kerstin. / Reduction of dual-species biofilm after sonic- or ultrasonic-activated irrigation protocols : A laboratory study. In: International Endodontic Journal. 2021 ; Vol. 54, No. 12. pp. 2219-2228.

Bibtex

@article{83f1f38ec67b46bdba8fc88342177b57,
title = "Reduction of dual-species biofilm after sonic- or ultrasonic-activated irrigation protocols: A laboratory study",
abstract = "AIM: To evaluate the antibacterial effect of sonic- and ultrasonic-activated irrigation on bacterial reduction of a dual-species biofilm in root canals compared to nonactivated irrigation in a laboratory study.METHODOLOGY: Two hundred and forty extracted human single-rooted maxillary anterior teeth were divided into two main groups (G, n = 120) according to the initial preparation size of the root canal (G1: size 25, 0.06 taper, G2: size 40, 0.06 taper). Root canals were inoculated with Enterococcus faecalis and Streptococcus oralis. After 5 days, G1 received combined instrumentation (up to size 40, 0.06 taper) and irrigation/activation, whereas G2 received solely irrigation/activation protocols. In both groups, irrigation was performed with sodium hypochlorite (NaOCl 1%) or physiological saline (NaCl 0.9%), using nonactivated syringe irrigation, sonic activation (2 x 30 s) or ultrasonic activation (2 x 30 s). Logarithmic reduction factors (LRFs) of colony-forming units were analysed separately for dentine-adherent and planktonic bacteria immediately after irrigation/activation protocols (time-point 1) or after 5 days of further incubation (time-point 2) by analysis of variance (anova) and post hoc tests (Tukey's HSD, t-test). The significance level was set at 0.05.RESULTS: In G1 subgroups (combined instrumentation with irrigation/activation), LRFs were significantly affected by the applied irrigation solution (p < .0001), but not by the activation method (p > .05; anova). In G2 subgroups (solely irrigation/activation), both, irrigant solution and activation, significantly affected LRFs (p < .0001, anova). Sonic activation resulted in significantly higher LRFs than ultrasonic activation (p < .0001) which had significantly greater reductions than nonactivated irrigation (p < .05; Tukey's HSD). At T2, strong bacterial regrowth was observed in all groups; however, a significant bacterial reduction was detected for factors instrumentation, irrigant solution and activation (p < .0001; anova). Similar LRFs were found for dentine-adherent and planktonic bacterial cells in all groups (r = 0.91 at T1, r = 0.8 at T2).CONCLUSIONS: In this laboratory study on extracted maxillary anterior teeth high-frequency sonic activation resulted in a greater bacterial reduction compared to ultrasonic activation in groups receiving solely irrigation/activation protocols; however, irrigation using NaOCl and ultrasonic activation also contributed significantly to bacterial reduction compared to the control groups.",
keywords = "antibacterial effectiveness, nonactivated irrigation, root canal disinfection, sonic activation, ultrasonic activation, BACTERIA, ROOT, AGITATION TECHNIQUES, ENTEROCOCCUS-FAECALIS, TEETH, SODIUM-HYPOCHLORITE, REMOVAL, DENTINAL TUBULES, ANTIBACTERIAL EFFICACY, APICAL PERIODONTITIS",
author = "Daniela Hoedke and Namira Kaulika and Henrik Dommisch and Sebastian Schlafer and Hagay Shemesh and Kerstin Bitter",
note = "{\textcopyright} 2021 The Authors. International Endodontic Journal published by John Wiley & Sons Ltd on behalf of British Endodontic Society.",
year = "2021",
month = dec,
doi = "10.1111/iej.13618",
language = "English",
volume = "54",
pages = "2219--2228",
journal = "International Endodontic Journal Online",
issn = "1365-2591",
publisher = "Wiley-Blackwell Publishing Ltd.",
number = "12",

}

RIS

TY - JOUR

T1 - Reduction of dual-species biofilm after sonic- or ultrasonic-activated irrigation protocols

T2 - A laboratory study

AU - Hoedke, Daniela

AU - Kaulika, Namira

AU - Dommisch, Henrik

AU - Schlafer, Sebastian

AU - Shemesh, Hagay

AU - Bitter, Kerstin

N1 - © 2021 The Authors. International Endodontic Journal published by John Wiley & Sons Ltd on behalf of British Endodontic Society.

PY - 2021/12

Y1 - 2021/12

N2 - AIM: To evaluate the antibacterial effect of sonic- and ultrasonic-activated irrigation on bacterial reduction of a dual-species biofilm in root canals compared to nonactivated irrigation in a laboratory study.METHODOLOGY: Two hundred and forty extracted human single-rooted maxillary anterior teeth were divided into two main groups (G, n = 120) according to the initial preparation size of the root canal (G1: size 25, 0.06 taper, G2: size 40, 0.06 taper). Root canals were inoculated with Enterococcus faecalis and Streptococcus oralis. After 5 days, G1 received combined instrumentation (up to size 40, 0.06 taper) and irrigation/activation, whereas G2 received solely irrigation/activation protocols. In both groups, irrigation was performed with sodium hypochlorite (NaOCl 1%) or physiological saline (NaCl 0.9%), using nonactivated syringe irrigation, sonic activation (2 x 30 s) or ultrasonic activation (2 x 30 s). Logarithmic reduction factors (LRFs) of colony-forming units were analysed separately for dentine-adherent and planktonic bacteria immediately after irrigation/activation protocols (time-point 1) or after 5 days of further incubation (time-point 2) by analysis of variance (anova) and post hoc tests (Tukey's HSD, t-test). The significance level was set at 0.05.RESULTS: In G1 subgroups (combined instrumentation with irrigation/activation), LRFs were significantly affected by the applied irrigation solution (p < .0001), but not by the activation method (p > .05; anova). In G2 subgroups (solely irrigation/activation), both, irrigant solution and activation, significantly affected LRFs (p < .0001, anova). Sonic activation resulted in significantly higher LRFs than ultrasonic activation (p < .0001) which had significantly greater reductions than nonactivated irrigation (p < .05; Tukey's HSD). At T2, strong bacterial regrowth was observed in all groups; however, a significant bacterial reduction was detected for factors instrumentation, irrigant solution and activation (p < .0001; anova). Similar LRFs were found for dentine-adherent and planktonic bacterial cells in all groups (r = 0.91 at T1, r = 0.8 at T2).CONCLUSIONS: In this laboratory study on extracted maxillary anterior teeth high-frequency sonic activation resulted in a greater bacterial reduction compared to ultrasonic activation in groups receiving solely irrigation/activation protocols; however, irrigation using NaOCl and ultrasonic activation also contributed significantly to bacterial reduction compared to the control groups.

AB - AIM: To evaluate the antibacterial effect of sonic- and ultrasonic-activated irrigation on bacterial reduction of a dual-species biofilm in root canals compared to nonactivated irrigation in a laboratory study.METHODOLOGY: Two hundred and forty extracted human single-rooted maxillary anterior teeth were divided into two main groups (G, n = 120) according to the initial preparation size of the root canal (G1: size 25, 0.06 taper, G2: size 40, 0.06 taper). Root canals were inoculated with Enterococcus faecalis and Streptococcus oralis. After 5 days, G1 received combined instrumentation (up to size 40, 0.06 taper) and irrigation/activation, whereas G2 received solely irrigation/activation protocols. In both groups, irrigation was performed with sodium hypochlorite (NaOCl 1%) or physiological saline (NaCl 0.9%), using nonactivated syringe irrigation, sonic activation (2 x 30 s) or ultrasonic activation (2 x 30 s). Logarithmic reduction factors (LRFs) of colony-forming units were analysed separately for dentine-adherent and planktonic bacteria immediately after irrigation/activation protocols (time-point 1) or after 5 days of further incubation (time-point 2) by analysis of variance (anova) and post hoc tests (Tukey's HSD, t-test). The significance level was set at 0.05.RESULTS: In G1 subgroups (combined instrumentation with irrigation/activation), LRFs were significantly affected by the applied irrigation solution (p < .0001), but not by the activation method (p > .05; anova). In G2 subgroups (solely irrigation/activation), both, irrigant solution and activation, significantly affected LRFs (p < .0001, anova). Sonic activation resulted in significantly higher LRFs than ultrasonic activation (p < .0001) which had significantly greater reductions than nonactivated irrigation (p < .05; Tukey's HSD). At T2, strong bacterial regrowth was observed in all groups; however, a significant bacterial reduction was detected for factors instrumentation, irrigant solution and activation (p < .0001; anova). Similar LRFs were found for dentine-adherent and planktonic bacterial cells in all groups (r = 0.91 at T1, r = 0.8 at T2).CONCLUSIONS: In this laboratory study on extracted maxillary anterior teeth high-frequency sonic activation resulted in a greater bacterial reduction compared to ultrasonic activation in groups receiving solely irrigation/activation protocols; however, irrigation using NaOCl and ultrasonic activation also contributed significantly to bacterial reduction compared to the control groups.

KW - antibacterial effectiveness

KW - nonactivated irrigation

KW - root canal disinfection

KW - sonic activation

KW - ultrasonic activation

KW - BACTERIA

KW - ROOT

KW - AGITATION TECHNIQUES

KW - ENTEROCOCCUS-FAECALIS

KW - TEETH

KW - SODIUM-HYPOCHLORITE

KW - REMOVAL

KW - DENTINAL TUBULES

KW - ANTIBACTERIAL EFFICACY

KW - APICAL PERIODONTITIS

U2 - 10.1111/iej.13618

DO - 10.1111/iej.13618

M3 - Journal article

C2 - 34418114

VL - 54

SP - 2219

EP - 2228

JO - International Endodontic Journal Online

JF - International Endodontic Journal Online

SN - 1365-2591

IS - 12

ER -