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Reconstitution of Na,K-ATPase in Nanodiscs

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Nanodiscs are disc-shaped self-assembled lipid bilayers encircled by membrane scaffolding proteins derived from Apolipoprotein A-1 (apo A-1). They constitute a versatile tool for studying membrane proteins since reconstitution into nanodiscs allows studies of the membrane proteins in detergent-free aqueous solutions in a lipid bilayer. Here, we apply the technique to the Na +,K +-ATPase (NKA) from pig kidney using Membrane Scaffolding Protein 1 D1 (MSP1D1). Contrary to other reports, the nanodiscs obtained by our protocol are built up of the native lipids originally present in the detergent solubilized sample together with the NKA.

Original languageEnglish
JournalMethods in Molecular Biology
Pages (from-to)403-409
Number of pages7
Publication statusPublished - 2016

    Research areas

  • Na ,K -ATPase, Nanodisc, Native lipids, Scaffolding protein, Self-assembled lipid bilayer, Self-assembly

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