Reconstitution of Na,K-ATPase in Nanodiscs

Jonas Lindholt Gregersen; Natalya Fedosova; Poul Nissen; Thomas Boesen

Reconstitution of Na,K-ATPase in Nanodiscs

Jonas Lindholt Gregersen, Natalya Fedosova, Poul Nissen, Thomas Boesen

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

6 Citations (Scopus)

Abstract

Nanodiscs are disc-shaped self-assembled lipid bilayers encircled by membrane scaffolding proteins derived from Apolipoprotein A-1 (apo A-1). They constitute a versatile tool for studying membrane proteins since reconstitution into nanodiscs allows studies of the membrane proteins in detergent-free aqueous solutions in a lipid bilayer. Here, we apply the technique to the Na ⁺,K ⁺-ATPase (NKA) from pig kidney using Membrane Scaffolding Protein 1 D1 (MSP1D1). Contrary to other reports, the nanodiscs obtained by our protocol are built up of the native lipids originally present in the detergent solubilized sample together with the NKA.

Original language	English
Journal	Methods in Molecular Biology
Volume	1377
Pages (from-to)	403-409
Number of pages	7
Publication status	Published - 1 Jan 2016

Keywords

Na ,K -ATPase
Nanodisc
Native lipids
Scaffolding protein
Self-assembled lipid bilayer
Self-assembly

Cite this

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title = "Reconstitution of Na,K-ATPase in Nanodiscs",

abstract = "Nanodiscs are disc-shaped self-assembled lipid bilayers encircled by membrane scaffolding proteins derived from Apolipoprotein A-1 (apo A-1). They constitute a versatile tool for studying membrane proteins since reconstitution into nanodiscs allows studies of the membrane proteins in detergent-free aqueous solutions in a lipid bilayer. Here, we apply the technique to the Na +,K +-ATPase (NKA) from pig kidney using Membrane Scaffolding Protein 1 D1 (MSP1D1). Contrary to other reports, the nanodiscs obtained by our protocol are built up of the native lipids originally present in the detergent solubilized sample together with the NKA.",

keywords = "Na ,K -ATPase, Nanodisc, Native lipids, Scaffolding protein, Self-assembled lipid bilayer, Self-assembly",

author = "Gregersen, {Jonas Lindholt} and Natalya Fedosova and Poul Nissen and Thomas Boesen",

year = "2016",

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journal = "Methods in Molecular Biology",

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T1 - Reconstitution of Na,K-ATPase in Nanodiscs

AU - Gregersen, Jonas Lindholt

AU - Fedosova, Natalya

AU - Nissen, Poul

AU - Boesen, Thomas

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Nanodiscs are disc-shaped self-assembled lipid bilayers encircled by membrane scaffolding proteins derived from Apolipoprotein A-1 (apo A-1). They constitute a versatile tool for studying membrane proteins since reconstitution into nanodiscs allows studies of the membrane proteins in detergent-free aqueous solutions in a lipid bilayer. Here, we apply the technique to the Na +,K +-ATPase (NKA) from pig kidney using Membrane Scaffolding Protein 1 D1 (MSP1D1). Contrary to other reports, the nanodiscs obtained by our protocol are built up of the native lipids originally present in the detergent solubilized sample together with the NKA.

AB - Nanodiscs are disc-shaped self-assembled lipid bilayers encircled by membrane scaffolding proteins derived from Apolipoprotein A-1 (apo A-1). They constitute a versatile tool for studying membrane proteins since reconstitution into nanodiscs allows studies of the membrane proteins in detergent-free aqueous solutions in a lipid bilayer. Here, we apply the technique to the Na +,K +-ATPase (NKA) from pig kidney using Membrane Scaffolding Protein 1 D1 (MSP1D1). Contrary to other reports, the nanodiscs obtained by our protocol are built up of the native lipids originally present in the detergent solubilized sample together with the NKA.

KW - Na ,K -ATPase

KW - Nanodisc

KW - Native lipids

KW - Scaffolding protein

KW - Self-assembled lipid bilayer

KW - Self-assembly

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M3 - Journal article

SN - 1940-6029

VL - 1377

SP - 403

EP - 409

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Reconstitution of Na,K-ATPase in Nanodiscs

Abstract

Keywords

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