Recombinant adeno-associated virus mediated gene delivery in the extracranial nervous system of adult mice by direct nerve immersion

Mette Richner*, Nádia Pereira Gonçalves, Poul Henning Jensen, Jens Randel Nyengaard, Christian Bjerggaard Vægter, Asad Jan*

*Corresponding author for this work

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Abstract

This protocol outlines a minimally invasive and quickly performed approach for transgene delivery in the extracranial nervous system of adult mice using recombinant adeno-associated virus (AAV). The technique, named Sciatic Nerve Direct Immersion (SciNDi), relies on the direct bilateral immersion of the exposed sciatic nerve with AAV. We show that in comparison with intramuscular AAV delivery, SciNDi results in widespread transduction in connected neuroanatomical tracts both in the sciatic nerve trunk and the lumbar spinal cord. For complete details on the use and execution of this protocol, please refer to Jan et al. (2019) and Richner et al. (2011, 2017).

Original languageEnglish
Article number101181
JournalSTAR Protocols
Volume3
Issue1
Number of pages15
DOIs
Publication statusPublished - Mar 2022

Keywords

  • Gene Expression
  • Microscopy
  • Model Organisms
  • Neuroscience

Fingerprint

Dive into the research topics of 'Recombinant adeno-associated virus mediated gene delivery in the extracranial nervous system of adult mice by direct nerve immersion'. Together they form a unique fingerprint.

Cite this