Ratjadones inhibit nuclear export by blocking CRM1/exportin 1

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Standard

Ratjadones inhibit nuclear export by blocking CRM1/exportin 1. / Köster, Mario; Lykke-Andersen, Søren; Elnakady, Yasser A; Gerth, Klaus; Washausen, Peter; Höfle, Gerhard; Sasse, Florenz; Kjems, Jørgen; Hauser, Hansjörg.

In: Experimental Cell Research, Vol. 286, No. 2, 2003, p. 321-331.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Köster, M, Lykke-Andersen, S, Elnakady, YA, Gerth, K, Washausen, P, Höfle, G, Sasse, F, Kjems, J & Hauser, H 2003, 'Ratjadones inhibit nuclear export by blocking CRM1/exportin 1', Experimental Cell Research, vol. 286, no. 2, pp. 321-331. https://doi.org/10.1016/S0014-4827(03)00100-9

APA

Köster, M., Lykke-Andersen, S., Elnakady, Y. A., Gerth, K., Washausen, P., Höfle, G., ... Hauser, H. (2003). Ratjadones inhibit nuclear export by blocking CRM1/exportin 1. Experimental Cell Research, 286(2), 321-331. https://doi.org/10.1016/S0014-4827(03)00100-9

CBE

Köster M, Lykke-Andersen S, Elnakady YA, Gerth K, Washausen P, Höfle G, Sasse F, Kjems J, Hauser H. 2003. Ratjadones inhibit nuclear export by blocking CRM1/exportin 1. Experimental Cell Research. 286(2):321-331. https://doi.org/10.1016/S0014-4827(03)00100-9

MLA

Köster, Mario et al. "Ratjadones inhibit nuclear export by blocking CRM1/exportin 1". Experimental Cell Research. 2003, 286(2). 321-331. https://doi.org/10.1016/S0014-4827(03)00100-9

Vancouver

Köster M, Lykke-Andersen S, Elnakady YA, Gerth K, Washausen P, Höfle G et al. Ratjadones inhibit nuclear export by blocking CRM1/exportin 1. Experimental Cell Research. 2003;286(2):321-331. https://doi.org/10.1016/S0014-4827(03)00100-9

Author

Köster, Mario ; Lykke-Andersen, Søren ; Elnakady, Yasser A ; Gerth, Klaus ; Washausen, Peter ; Höfle, Gerhard ; Sasse, Florenz ; Kjems, Jørgen ; Hauser, Hansjörg. / Ratjadones inhibit nuclear export by blocking CRM1/exportin 1. In: Experimental Cell Research. 2003 ; Vol. 286, No. 2. pp. 321-331.

Bibtex

@article{cbae34d163234f72925de7522894d0de,
title = "Ratjadones inhibit nuclear export by blocking CRM1/exportin 1",
abstract = "In addition to previously isolated ratjadone A we describe three new members of this family, ratjadones B, C, and D, from another strain of the myxobacterium Sorangium cellulosum. We have investigated the properties of these ratjadones with respect to their activity on mammalian cell lines. We found IC(50) values in the picomolar range and a significant increase in the size of nuclei. A further examination showed that they inhibit the export of the leucine-rich nuclear export signal (LR-NES) containing proteins in different cell lines. Ratjadones are able to inhibit the formation of the nuclear export complex composed of the CRM1, RanGTP, and the cargo protein, as shown by two different in vitro assays. Finally, the binding of ratjadone C to CRM1 was demonstrated. These ratjadone activities are in the same concentration range as described for the polyketide leptomycin B (LMB) from Streptomyces sp. Like LMB, it seems that the ratjadones covalently bind to CRM1, inhibit cargo protein binding via LR-NES, and thereby block nuclear export. Thus, the ratjadones represent a new class of natural compounds which inhibit proliferation in eukaryotes by blocking nuclear export.",
keywords = "3T3 Cells, Active Transport, Cell Nucleus, Animals, Cell Division, Cell Nucleus, Cell Size, Dose-Response Relationship, Drug, Eukaryotic Cells, Karyopherins, Macromolecular Substances, Mice, Molecular Structure, Myxococcales, Protein Binding, Protein Sorting Signals, Protein Transport, Pyrones, Receptors, Cytoplasmic and Nuclear, Signal Transduction, ran GTP-Binding Protein",
author = "Mario K{\"o}ster and S{\o}ren Lykke-Andersen and Elnakady, {Yasser A} and Klaus Gerth and Peter Washausen and Gerhard H{\"o}fle and Florenz Sasse and J{\o}rgen Kjems and Hansj{\"o}rg Hauser",
year = "2003",
doi = "10.1016/S0014-4827(03)00100-9",
language = "English",
volume = "286",
pages = "321--331",
journal = "Experimental Cell Research",
issn = "0014-4827",
publisher = "Academic Press",
number = "2",

}

RIS

TY - JOUR

T1 - Ratjadones inhibit nuclear export by blocking CRM1/exportin 1

AU - Köster, Mario

AU - Lykke-Andersen, Søren

AU - Elnakady, Yasser A

AU - Gerth, Klaus

AU - Washausen, Peter

AU - Höfle, Gerhard

AU - Sasse, Florenz

AU - Kjems, Jørgen

AU - Hauser, Hansjörg

PY - 2003

Y1 - 2003

N2 - In addition to previously isolated ratjadone A we describe three new members of this family, ratjadones B, C, and D, from another strain of the myxobacterium Sorangium cellulosum. We have investigated the properties of these ratjadones with respect to their activity on mammalian cell lines. We found IC(50) values in the picomolar range and a significant increase in the size of nuclei. A further examination showed that they inhibit the export of the leucine-rich nuclear export signal (LR-NES) containing proteins in different cell lines. Ratjadones are able to inhibit the formation of the nuclear export complex composed of the CRM1, RanGTP, and the cargo protein, as shown by two different in vitro assays. Finally, the binding of ratjadone C to CRM1 was demonstrated. These ratjadone activities are in the same concentration range as described for the polyketide leptomycin B (LMB) from Streptomyces sp. Like LMB, it seems that the ratjadones covalently bind to CRM1, inhibit cargo protein binding via LR-NES, and thereby block nuclear export. Thus, the ratjadones represent a new class of natural compounds which inhibit proliferation in eukaryotes by blocking nuclear export.

AB - In addition to previously isolated ratjadone A we describe three new members of this family, ratjadones B, C, and D, from another strain of the myxobacterium Sorangium cellulosum. We have investigated the properties of these ratjadones with respect to their activity on mammalian cell lines. We found IC(50) values in the picomolar range and a significant increase in the size of nuclei. A further examination showed that they inhibit the export of the leucine-rich nuclear export signal (LR-NES) containing proteins in different cell lines. Ratjadones are able to inhibit the formation of the nuclear export complex composed of the CRM1, RanGTP, and the cargo protein, as shown by two different in vitro assays. Finally, the binding of ratjadone C to CRM1 was demonstrated. These ratjadone activities are in the same concentration range as described for the polyketide leptomycin B (LMB) from Streptomyces sp. Like LMB, it seems that the ratjadones covalently bind to CRM1, inhibit cargo protein binding via LR-NES, and thereby block nuclear export. Thus, the ratjadones represent a new class of natural compounds which inhibit proliferation in eukaryotes by blocking nuclear export.

KW - 3T3 Cells

KW - Active Transport, Cell Nucleus

KW - Animals

KW - Cell Division

KW - Cell Nucleus

KW - Cell Size

KW - Dose-Response Relationship, Drug

KW - Eukaryotic Cells

KW - Karyopherins

KW - Macromolecular Substances

KW - Mice

KW - Molecular Structure

KW - Myxococcales

KW - Protein Binding

KW - Protein Sorting Signals

KW - Protein Transport

KW - Pyrones

KW - Receptors, Cytoplasmic and Nuclear

KW - Signal Transduction

KW - ran GTP-Binding Protein

U2 - 10.1016/S0014-4827(03)00100-9

DO - 10.1016/S0014-4827(03)00100-9

M3 - Journal article

C2 - 12749860

VL - 286

SP - 321

EP - 331

JO - Experimental Cell Research

JF - Experimental Cell Research

SN - 0014-4827

IS - 2

ER -