Quantitative proteomics and bioinformatic analysis provide new insight into the dynamic response of porcine intestine to Salmonella Typhimurium

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DOI

  • Melanie Collado-Romero, Univ Cordoba, Universidad de Cordoba, Dept Genet, Fac Vet, Grp Genom & Mejora Anim
  • ,
  • Carmen Aguilar, Univ Cordoba, Universidad de Cordoba, Dept Genet, Fac Vet, Grp Genom & Mejora Anim, Denmark
  • Cristina Arce, Univ Cordoba, Universidad de Cordoba, Fac Vet, Dept Anim Prod, Denmark
  • Concepcion Lucena, Univ Cordoba, Universidad de Cordoba, Dept Genet, Fac Vet, Grp Genom & Mejora Anim, Denmark
  • Marius C. Codrea
  • ,
  • Luis Morera, Univ Cordoba, Universidad de Cordoba, Dept Genet, Fac Vet, Grp Genom & Mejora Anim, Denmark
  • Emoke Bendixen
  • Angela Moreno, CSIC, Consejo Superior de Investigaciones Cientificas (CSIC), Inst Agr Sostenible, Denmark
  • Juan J. Garrido, Univ Cordoba, Universidad de Cordoba, Dept Genet, Fac Vet, Grp Genom & Mejora Anim

The enteropathogen Salmonella Typhimurium (S. Typhimurium) is the most commonly non-typhoideal serotype isolated in pig worldwide. Currently, one of the main sources of human infection is by consumption of pork meat. Therefore, prevention and control of salmonellosis in pigs is crucial for minimizing risks to public health. The aim of the present study was to use isobaric tags for relative and absolute quantification (iTRAQ) to explore differences in the response to Salmonella in two segment of the porcine gut (ileum and colon) along a time course of 1, 2, and 6 days post infection (dpi) with S. Typhimurium. A total of 298 proteins were identified in the infected ileum samples of which, 112 displayed significant expression differences due to Salmonella infection. In colon, 184 proteins were detected in the infected samples of which 46 resulted differentially expressed with respect to the controls. The higher number of changes in protein expression was quantified in ileum at 2 dpi. Further biological interpretation of proteomics data using bioinformatics tools demonstrated that the expression changes in colon were found in proteins involved in cell death and survival, tissue morphology or molecular transport at the early stages and tissue regeneration at 6 dpi. In ileum, however, changes in protein expression were mainly related to immunological and infection diseases, inflammatory response or connective tissue disorders at 1 and 2 dpi. iTRAQ has proved to be a proteomic robust approach allowing us to identify ileum as the earliest response focus upon S. Typhimurium in the porcine gut. In addition, new functions involved in the response to bacteria such as elF2 signaling, free radical scavengers or antimicrobial peptides (AMP) expression have been identified. Finally, the impairment at of the enterohepatic circulation of bile acids and lipid metabolism by means the under regulation of FABP6 protein and FXR/RXR and LXR/RXR signaling pathway in ileum has been established for the first time in pigs. Taken together, our results provide a better understanding of the porcine response to Salmonella infection and the molecular mechanisms underlying Salmonella-host interactions.

Original languageEnglish
Article number64
JournalFrontiers in cellular and infection microbiology
Volume5
Number of pages10
ISSN2235-2988
DOIs
Publication statusPublished - 3 Sep 2015

    Research areas

  • iTRAQ, Salmonella Typhimurium, experimentally infected pigs, intestinal response, ileum, colon, ACID-BINDING PROTEIN, NITRIC-OXIDE, EPITHELIAL-CELLS, INNATE IMMUNITY, HOST-DEFENSE, ACTIVATION, EXPRESSION, INHIBITION, STRESS, MUCOSA

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