Quantitative Detection of Digoxin in Plasma Using Small-Molecule Immunoassay in a Recyclable Gravity-Driven Microfluidic Chip

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Quantitative Detection of Digoxin in Plasma Using Small-Molecule Immunoassay in a Recyclable Gravity-Driven Microfluidic Chip. / Li, Hailong; Vinther, Jesper; Gothelf, Kurt Vesterager.

In: Advanced Science, Vol. 6, No. 6, 1802051, 2019.

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@article{da9b614d1cbb4c08ba2469ba66dc059e,
title = "Quantitative Detection of Digoxin in Plasma Using Small-Molecule Immunoassay in a Recyclable Gravity-Driven Microfluidic Chip",
abstract = "Immunoassays are critical for clinical diagnostics and biomedical research. However, two major challenges remaining in conventional immunoassays are precise quantification and development of immunoassays for small-molecule detection. Here, a two signal-mode small-molecule immunoassay containing an internal reference that provides high stability and reproducibility compared to conventional small-molecule immunoassays is presented. A system is developed for quantitative monitoring of the digoxin concentration in plasma in the clinically relevant range (0.6–2.6 nm). Furthermore, the model system is integrated into a simple gravity-driven microfluidic chip (G-Chip) requiring only 10 µL plasma. The G-Chip allows fast detection without any complex operation and can be recycled for at least 50 times. The assay, and the G-Chip in particular, has the potential for further development of point-of-care (POC) diagnostics.",
keywords = "CONJUGATION, DNA, RESIDUES, beads, digoxin, gravity-driven microfluidic chips, two signal-mode immunoassays",
author = "Hailong Li and Jesper Vinther and Gothelf, {Kurt Vesterager}",
year = "2019",
doi = "10.1002/advs.201802051",
language = "English",
volume = "6",
journal = "Advanced Science",
issn = "2198-3844",
publisher = "Wiley",
number = "6",

}

RIS

TY - JOUR

T1 - Quantitative Detection of Digoxin in Plasma Using Small-Molecule Immunoassay in a Recyclable Gravity-Driven Microfluidic Chip

AU - Li, Hailong

AU - Vinther, Jesper

AU - Gothelf, Kurt Vesterager

PY - 2019

Y1 - 2019

N2 - Immunoassays are critical for clinical diagnostics and biomedical research. However, two major challenges remaining in conventional immunoassays are precise quantification and development of immunoassays for small-molecule detection. Here, a two signal-mode small-molecule immunoassay containing an internal reference that provides high stability and reproducibility compared to conventional small-molecule immunoassays is presented. A system is developed for quantitative monitoring of the digoxin concentration in plasma in the clinically relevant range (0.6–2.6 nm). Furthermore, the model system is integrated into a simple gravity-driven microfluidic chip (G-Chip) requiring only 10 µL plasma. The G-Chip allows fast detection without any complex operation and can be recycled for at least 50 times. The assay, and the G-Chip in particular, has the potential for further development of point-of-care (POC) diagnostics.

AB - Immunoassays are critical for clinical diagnostics and biomedical research. However, two major challenges remaining in conventional immunoassays are precise quantification and development of immunoassays for small-molecule detection. Here, a two signal-mode small-molecule immunoassay containing an internal reference that provides high stability and reproducibility compared to conventional small-molecule immunoassays is presented. A system is developed for quantitative monitoring of the digoxin concentration in plasma in the clinically relevant range (0.6–2.6 nm). Furthermore, the model system is integrated into a simple gravity-driven microfluidic chip (G-Chip) requiring only 10 µL plasma. The G-Chip allows fast detection without any complex operation and can be recycled for at least 50 times. The assay, and the G-Chip in particular, has the potential for further development of point-of-care (POC) diagnostics.

KW - CONJUGATION

KW - DNA

KW - RESIDUES

KW - beads

KW - digoxin

KW - gravity-driven microfluidic chips

KW - two signal-mode immunoassays

UR - http://www.scopus.com/inward/record.url?scp=85060737532&partnerID=8YFLogxK

U2 - 10.1002/advs.201802051

DO - 10.1002/advs.201802051

M3 - Journal article

C2 - 30937271

VL - 6

JO - Advanced Science

JF - Advanced Science

SN - 2198-3844

IS - 6

M1 - 1802051

ER -