Quantitative analysis of absorption, metabolism, and excretion of benzoxazinoids in humans after the consumption of high- and low-benzoxazinoid diets with similar contents of cereal dietary fibres: a crossover study

TY - JOUR

T1 - Quantitative analysis of absorption, metabolism, and excretion of benzoxazinoids in humans after the consumption of high- and low-benzoxazinoid diets with similar contents of cereal dietary fibres: a crossover study

AU - Jensen, Bettina M.

AU - B. Adhikari, Khem

AU - Schnoor, Heidi J.

AU - Juel-Berg, Nanna

AU - Fomsgaard, Inge S.

AU - Poulsen, Lars K.

PY - 2017/2/1

Y1 - 2017/2/1

N2 - Purpose: Benzoxazinoids (BXs) are a group of wholegrain phytochemicals with potential pharmacological properties; however, limited information exists on their absorption, metabolism, and excretion in humans. The aim of this study was to investigate the dose-dependent uptake and excretion of dietary BXs in a healthy population. Methods: Blood and urine were collected from 19 healthy participants from a crossover study after a washout, a LOW BX diet or HIGH BX diet, and analysed for 12 BXs and 4 phenoxazinone derivatives. Results: We found that the plasma BX level peaked approximately 3 h after food intake, whereas BXs in urine were present even at 36 h after consuming a meal. No phenoxazinone derivatives could be detected in either plasma or urine. The dominant BX metabolite in both plasma and urine was 2-β-d-glucopyranosyloxy-1,4-benzoxazin-3-one (HBOA-Glc), even though 2-β-d-glucopyranosyloxy-4-hydroxy-1,4-benzoxazin-3-one (DIBOA-Glc) was the major component in the diet. Conclusion: The dietary BX treatment correlated well with the plasma and urine levels, illustrating strong dose-dependent BX absorption, which also had a rapid washout, especially from the plasma compartment.

AB - Purpose: Benzoxazinoids (BXs) are a group of wholegrain phytochemicals with potential pharmacological properties; however, limited information exists on their absorption, metabolism, and excretion in humans. The aim of this study was to investigate the dose-dependent uptake and excretion of dietary BXs in a healthy population. Methods: Blood and urine were collected from 19 healthy participants from a crossover study after a washout, a LOW BX diet or HIGH BX diet, and analysed for 12 BXs and 4 phenoxazinone derivatives. Results: We found that the plasma BX level peaked approximately 3 h after food intake, whereas BXs in urine were present even at 36 h after consuming a meal. No phenoxazinone derivatives could be detected in either plasma or urine. The dominant BX metabolite in both plasma and urine was 2-β-d-glucopyranosyloxy-1,4-benzoxazin-3-one (HBOA-Glc), even though 2-β-d-glucopyranosyloxy-4-hydroxy-1,4-benzoxazin-3-one (DIBOA-Glc) was the major component in the diet. Conclusion: The dietary BX treatment correlated well with the plasma and urine levels, illustrating strong dose-dependent BX absorption, which also had a rapid washout, especially from the plasma compartment.

KW - benzoxazinoids

KW - metabolism

KW - rye bread

KW - weight loss

KW - wholegrain

UR - https://www.scopus.com/pages/publications/84945542296

U2 - 10.1007/s00394-015-1088-6

DO - 10.1007/s00394-015-1088-6

M3 - Journal article

C2 - 26519282

SN - 1436-6207

VL - 56

SP - 387

EP - 397

JO - European Journal of Nutrition

JF - European Journal of Nutrition

IS - 1

ER -