Abstract
Background: There is an imbalance between organ supply and organ demand for kidney transplantation, which could be reduced by accepting more marginal kidneys. Optical imaging technologies enable quantification of microperfusion, microvascularisation and microanatomy. Due to their high resolution and non-invasive performance, these imaging technologies are promising methods for improving the evaluation of kidney viability in more details before transplantation.
Materials and methods: Nephrectomised porcine kidneys are connected to an ex vivo machine perfusion system. The cortical perfusion is gradually reduced by ex vivo embolization based on microsphere injection. A tissue-perfusion and oxygen sensor are positioned in the cortex for direct reference measurements of relative changes in cortical perfusion and pO2. Optical imaging is employed to the kidney, including Laser Speckle Contrast Imaging (LSCI), Fluorescence Based Imaging (FBI) and Optical Coherence Tomography (OCT).
Expected outcome: This study seeks to establish novel methods to improve kidney viability evaluation before transplantation. The aim of this project is to quantify kidney microperfusion and microvascularisation using LSCI, FBI and OCT, and furthermore, to validate these measurements. Today, no results have been produced, but acquired data will be collected and analysed from December 2022.
Conclusion: Our ambition is to establish novel methods to improve kidney viability evaluation before transplantation. By performing this study, we expect to validate optical imaging methods for the quantification of kidney microperfusion and microvascularisation.
Materials and methods: Nephrectomised porcine kidneys are connected to an ex vivo machine perfusion system. The cortical perfusion is gradually reduced by ex vivo embolization based on microsphere injection. A tissue-perfusion and oxygen sensor are positioned in the cortex for direct reference measurements of relative changes in cortical perfusion and pO2. Optical imaging is employed to the kidney, including Laser Speckle Contrast Imaging (LSCI), Fluorescence Based Imaging (FBI) and Optical Coherence Tomography (OCT).
Expected outcome: This study seeks to establish novel methods to improve kidney viability evaluation before transplantation. The aim of this project is to quantify kidney microperfusion and microvascularisation using LSCI, FBI and OCT, and furthermore, to validate these measurements. Today, no results have been produced, but acquired data will be collected and analysed from December 2022.
Conclusion: Our ambition is to establish novel methods to improve kidney viability evaluation before transplantation. By performing this study, we expect to validate optical imaging methods for the quantification of kidney microperfusion and microvascularisation.
| Original language | English |
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| Publication date | 11 Mar 2023 |
| Publication status | Published - 11 Mar 2023 |
| Event | 14. Kongres for Medicinsk Studenterforskning - Sandbjerg Gods, Sønderborg, Denmark Duration: 9 Mar 2023 → 12 Mar 2023 Conference number: 14 https://studenterforskning.dk/studenterkongres/ |
Conference
| Conference | 14. Kongres for Medicinsk Studenterforskning |
|---|---|
| Number | 14 |
| Location | Sandbjerg Gods |
| Country/Territory | Denmark |
| City | Sønderborg |
| Period | 09/03/2023 → 12/03/2023 |
| Internet address |