You are here:
» Quantification of biofilm biomass by staining
About Aarhus University
Quantification of biofilm biomass by staining: Non-toxic safranin can replace the popular crystal violet
Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review
DOI
- https://doi.org/10.1016/j.mimet.2017.08.003
Final published version
- Pernille Ommen
- Natalia Zobek ,
- Rikke Louise Meyer
Crystal violet staining is commonly used for quantification of biofilm formation, although it is highly toxic. Here we test safranin as a non-toxic replacement. Safranin staining provided similar results as crystal violet, but with higher reproducibility. We therefore recommend safranin staining for biofilm biomass quantification.
| Original language | English |
|---|---|
| Journal | Journal of Microbiological Methods |
| Volume | 141 |
| Pages (from-to) | 87-89 |
| Number of pages | 3 |
| ISSN | 0167-7012 |
| DOIs | |
| Publication status | Published - Oct 2017 |
- Biofilm quantification, Crystal violet, CV assay, Safranin, DNA
Research areas
See relations at Aarhus University Citationformats
ID: 117768339