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Quantification of biofilm biomass by staining: Non-toxic safranin can replace the popular crystal violet

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Crystal violet staining is commonly used for quantification of biofilm formation, although it is highly toxic. Here we test safranin as a non-toxic replacement. Safranin staining provided similar results as crystal violet, but with higher reproducibility. We therefore recommend safranin staining for biofilm biomass quantification.

Original languageEnglish
JournalJournal of Microbiological Methods
Pages (from-to)87-89
Number of pages3
Publication statusPublished - Oct 2017

    Research areas

  • Biofilm quantification, Crystal violet, CV assay, Safranin, DNA

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