Aarhus University Seal

Purification and characterization of extracellular superoxide dismutase in mouse lung

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

DOI

  • Cheryl L. Fattman, Univ. of Pittsburgh Medical Center
  • ,
  • Jan J. Enghild
  • James D. Crapo, National Jewish Medical and Research Center
  • ,
  • Lisa M. Schaefer, Univ. of Pittsburgh Medical Center
  • ,
  • Zuzana Valnickova
  • ,
  • Tim D. Oury, Univ. of Pittsburgh Medical Center

Extracellular superoxide dismutase (EC-SOD) is the major isozyme of SOD in arteries, but is also abundant in lungs. In particular, mouse lungs contain large amounts of EC-SOD compared to lungs in other mammals. This suggests that EC-SOD may have an amplified function in the mouse lung. This study describes the purification and characterization of mouse EC-SOD as well as its localization in mouse lung. Mouse EC-SOD exists primarily as a homotetramer composed of a pair of dimers linked through disulfide bonds present in the heparin-binding domains of each sub-unit. In addition, mouse EC-SOD can exist in active multimeric forms. We developed and utilized a polyclonal antibody to mouse EC-SOD to immunolocalize EC-SOD in mouse lung. EC-SOD labeling is strongest in the matrix of vessels, airways, and alveolar septa. This localization suggests that EC-SOD may have important functions in pulmonary biology, perhaps in the modulation of nitric oxide-dependent responses. (C) 2000 Academic Press.

Original languageEnglish
JournalBiochemical and Biophysical Research Communications
Volume275
Issue2
Pages (from-to)542-548
Number of pages7
ISSN0006-291X
DOIs
Publication statusPublished - 28 Aug 2000

    Research areas

  • Antioxidants, Arteries, Hypertension, Nitric oxide

See relations at Aarhus University Citationformats

ID: 136859869