Proteomics of desiccation tolerance during development and germination of maize embryos

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  • Hui Huang, Chinese Academy of Sciences, China
  • Ian Max Møller
  • Song-Quan Song, Chinese Academy of Sciences, China

Maize seeds were used to identify the key embryo proteins involved in
desiccation tolerance during development and germination. Immature maize
embryos (28N) during development and mature embryos imbibed for 72 h
(72HN) are desiccation sensitive. Mature maize embryos (52N) during
development are desiccation tolerant. Thiobarbituric acid reactive
substance and hydrogen peroxide contents decreased and increased with
acquisition and loss of desiccation tolerance, respectively. A total of
111 protein spots changed significantly (1.5 fold increase/decrease) in
desiccation-tolerant and -sensitive embryos before (28N, 52N and 72HN)
and after (28D, 52D and 72HD) dehydration. Nine pre-dominantly proteins,
17.4 kDa Class I heat shock protein 3, late embryogenesis abundant
protein EMB564, outer membrane protein, globulin 2, TPA:putative
cystatin, NBS-LRR resistance-like protein RGC456, stress responsive
protein, major allergen Bet v 1.01C and proteasome subunit alpha type 1,
accumulated during embryo maturation, decreased during germination and
increased in desiccation-tolerant embryos during desiccation. Two
proteins, Rhd6-like 2 and low-molecular-weight heat shock protein
precursor, showed the inverse pattern. We infer that these eleven
proteins are involved in seed desiccation tolerance. We conclude that
desiccation-tolerant embryos make more economical use of their resources
to accumulate protective molecules and antioxidant systems to deal with
maturation drying and desiccation treatment..

Original languageEnglish
JournalJournal of Proteomics
Volume75
Issue4
Pages (from-to)1247-1262
Number of pages16
ISSN1874-3919
DOIs
Publication statusPublished - Feb 2012

    Research areas

  • Desiccation tolerance, Development, Germination, Maize embryos, Proteomic analysis

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