Proteomic Analysis Reveals Different Involvement of Embryo and Endosperm Proteins during Aging of Yliangyou 2 Hybrid Rice Seeds

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DOI

  • Ying-Xue Zhang, Chinese Acad Sci, Chinese Academy of Sciences, Inst Bot, Key Lab Plant Resources
  • ,
  • Heng-Heng Xu, Chinese Acad Sci, Chinese Academy of Sciences, Inst Bot, Key Lab Plant Resources
  • ,
  • Shu-Jun Liu, Chinese Acad Sci, Chinese Academy of Sciences, Inst Bot, Key Lab Plant Resources
  • ,
  • Ni Li, Hunan Hybrid Rice Res Ctr, State Key Lab Hybrid Rice
  • ,
  • Wei-Qing Wang, Chinese Acad Sci, Chinese Academy of Sciences, Inst Bot, Key Lab Plant Resources
  • ,
  • Ian M. Moller
  • Song-Quan Song, Chinese Acad Sci, Chinese Academy of Sciences, Inst Bot, Key Lab Plant Resources

Seed aging is a process that results in a delayed germination, a decreased germination percentage, and finally a total loss of seed viability. However, the mechanism of seed aging is poorly understood. In the present study, Yliangyou 2 hybrid rice (Oryza sativa L.) seeds were artificially aged at 100% relative humidity and 40 degrees C, and the effect of artificial aging on germination, germination time course and the change in protein profiles of embryo and endosperm was studied to understand the molecular mechanism behind seed aging. With an increasing duration of artificial aging, the germination percentage and germination rate of hybrid rice seeds decreased. By comparing the protein profiles from the seeds aged for 0, 10 and 25 days, a total of 91 and 100 protein spots were found to show a significant change of more than 2-fold (P <0.05) in abundance, and 71 and 79 protein spots were identified, in embryos and endosperms, respectively. The great majority of these proteins increased in abundance in embryos (95%) and decreased in abundance in endosperms (99%). In embryos, most of the identified proteins were associated with energy (30%), with cell defense and rescue (28%), and with storage protein (18%). In endosperms, most of the identified proteins were involved in metabolism (37%), in energy (27%), and in protein synthesis and destination (11%). The most marked change was the increased abundance of many glycolytic enzymes together with the two fermentation enzymes pyruvate decarboxylase and alcohol dehydrogenase in the embryos during aging. We hypothesize that the decreased viability of hybrid rice seeds during artificial aging is caused by the development of hypoxic conditions in the embryos followed by ethanol accumulation.

Original languageEnglish
Article number1394
JournalFrontiers in Plant Science
Volume7
Number of pages17
ISSN1664-462X
DOIs
Publication statusPublished - 21 Sep 2016

    Research areas

  • Yliangyou 2 hybrid rice, proteome, embryo, endosperm, seed aging, WIDE CHARACTERIZATION, ARABIDOPSIS-THALIANA, SUGAR-BEET, SATIVA L., VIGOR, DETERIORATION, GERMINATION, BIOSYNTHESIS, EXPRESSION, MUTANTS

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