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Proteome analysis of pod and seed development in the model legume Lotus japonicus

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Standard

Proteome analysis of pod and seed development in the model legume Lotus japonicus. / Nautrup-Pedersen, Gitte; Dam, Svend; Laursen, Brian S; Siegumfeldt, Astrid L; Nielsen, Kasper; Goffard, Nicolas; Stærfeldt, Hans Henrik; Friis, Carsten; Sato, Shusei; Tabata, Satoshi; Lorentzen, Andrea; Roepstorff, Peter; Stougaard, Jens.

In: Journal of Proteome Research, Vol. 9, No. 11, 05.11.2010, p. 5715-26.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Nautrup-Pedersen, G, Dam, S, Laursen, BS, Siegumfeldt, AL, Nielsen, K, Goffard, N, Stærfeldt, HH, Friis, C, Sato, S, Tabata, S, Lorentzen, A, Roepstorff, P & Stougaard, J 2010, 'Proteome analysis of pod and seed development in the model legume Lotus japonicus', Journal of Proteome Research, vol. 9, no. 11, pp. 5715-26. https://doi.org/10.1021/pr100511u

APA

Nautrup-Pedersen, G., Dam, S., Laursen, B. S., Siegumfeldt, A. L., Nielsen, K., Goffard, N., ... Stougaard, J. (2010). Proteome analysis of pod and seed development in the model legume Lotus japonicus. Journal of Proteome Research, 9(11), 5715-26. https://doi.org/10.1021/pr100511u

CBE

Nautrup-Pedersen G, Dam S, Laursen BS, Siegumfeldt AL, Nielsen K, Goffard N, Stærfeldt HH, Friis C, Sato S, Tabata S, Lorentzen A, Roepstorff P, Stougaard J. 2010. Proteome analysis of pod and seed development in the model legume Lotus japonicus. Journal of Proteome Research. 9(11):5715-26. https://doi.org/10.1021/pr100511u

MLA

Nautrup-Pedersen, Gitte et al. "Proteome analysis of pod and seed development in the model legume Lotus japonicus". Journal of Proteome Research. 2010, 9(11). 5715-26. https://doi.org/10.1021/pr100511u

Vancouver

Nautrup-Pedersen G, Dam S, Laursen BS, Siegumfeldt AL, Nielsen K, Goffard N et al. Proteome analysis of pod and seed development in the model legume Lotus japonicus. Journal of Proteome Research. 2010 Nov 5;9(11):5715-26. https://doi.org/10.1021/pr100511u

Author

Nautrup-Pedersen, Gitte ; Dam, Svend ; Laursen, Brian S ; Siegumfeldt, Astrid L ; Nielsen, Kasper ; Goffard, Nicolas ; Stærfeldt, Hans Henrik ; Friis, Carsten ; Sato, Shusei ; Tabata, Satoshi ; Lorentzen, Andrea ; Roepstorff, Peter ; Stougaard, Jens. / Proteome analysis of pod and seed development in the model legume Lotus japonicus. In: Journal of Proteome Research. 2010 ; Vol. 9, No. 11. pp. 5715-26.

Bibtex

@article{7ec4972cf803487dabae7dc0d31664c8,
title = "Proteome analysis of pod and seed development in the model legume Lotus japonicus",
abstract = "Legume pods serve important functions during seed development and are themselves sources of food and feed. Compared to seeds, the metabolism and development of pods are not well-defined. The present characterization of pods from the model legume Lotus japonicus, together with the detailed analyses of the pod and seed proteomes in five developmental stages, paves the way for comparative pathway analysis and provides new metabolic information. Proteins were analyzed by two-dimensional gel electrophoresis and tandem-mass spectrometry. These analyses lead to the identification of 604 pod proteins and 965 seed proteins, including 263 proteins distinguishing the pod. The complete data set is publicly available at http://www.cbs.dtu.dk/cgi-bin/lotus/db.cgi , where spots in a reference map are linked to experimental data, such as matched peptides, quantification values, and gene accessions. Identified pod proteins represented enzymes from 85 different metabolic pathways, including storage globulins and a late embryogenesis abundant protein. In contrast to seed maturation, pod maturation was associated with decreasing total protein content, especially proteins involved in protein biosynthesis and photosynthesis. Proteins detected only in pods included three enzymes participating in the urea cycle and four in nitrogen and amino group metabolism, highlighting the importance of nitrogen metabolism during pod development. Additionally, five legume seed proteins previously unassigned in the glutamate metabolism pathway were identified.",
author = "Gitte Nautrup-Pedersen and Svend Dam and Laursen, {Brian S} and Siegumfeldt, {Astrid L} and Kasper Nielsen and Nicolas Goffard and St{\ae}rfeldt, {Hans Henrik} and Carsten Friis and Shusei Sato and Satoshi Tabata and Andrea Lorentzen and Peter Roepstorff and Jens Stougaard",
year = "2010",
month = "11",
day = "5",
doi = "10.1021/pr100511u",
language = "English",
volume = "9",
pages = "5715--26",
journal = "Journal of Proteome Research",
issn = "1535-3893",
publisher = "AMER CHEMICAL SOC",
number = "11",

}

RIS

TY - JOUR

T1 - Proteome analysis of pod and seed development in the model legume Lotus japonicus

AU - Nautrup-Pedersen, Gitte

AU - Dam, Svend

AU - Laursen, Brian S

AU - Siegumfeldt, Astrid L

AU - Nielsen, Kasper

AU - Goffard, Nicolas

AU - Stærfeldt, Hans Henrik

AU - Friis, Carsten

AU - Sato, Shusei

AU - Tabata, Satoshi

AU - Lorentzen, Andrea

AU - Roepstorff, Peter

AU - Stougaard, Jens

PY - 2010/11/5

Y1 - 2010/11/5

N2 - Legume pods serve important functions during seed development and are themselves sources of food and feed. Compared to seeds, the metabolism and development of pods are not well-defined. The present characterization of pods from the model legume Lotus japonicus, together with the detailed analyses of the pod and seed proteomes in five developmental stages, paves the way for comparative pathway analysis and provides new metabolic information. Proteins were analyzed by two-dimensional gel electrophoresis and tandem-mass spectrometry. These analyses lead to the identification of 604 pod proteins and 965 seed proteins, including 263 proteins distinguishing the pod. The complete data set is publicly available at http://www.cbs.dtu.dk/cgi-bin/lotus/db.cgi , where spots in a reference map are linked to experimental data, such as matched peptides, quantification values, and gene accessions. Identified pod proteins represented enzymes from 85 different metabolic pathways, including storage globulins and a late embryogenesis abundant protein. In contrast to seed maturation, pod maturation was associated with decreasing total protein content, especially proteins involved in protein biosynthesis and photosynthesis. Proteins detected only in pods included three enzymes participating in the urea cycle and four in nitrogen and amino group metabolism, highlighting the importance of nitrogen metabolism during pod development. Additionally, five legume seed proteins previously unassigned in the glutamate metabolism pathway were identified.

AB - Legume pods serve important functions during seed development and are themselves sources of food and feed. Compared to seeds, the metabolism and development of pods are not well-defined. The present characterization of pods from the model legume Lotus japonicus, together with the detailed analyses of the pod and seed proteomes in five developmental stages, paves the way for comparative pathway analysis and provides new metabolic information. Proteins were analyzed by two-dimensional gel electrophoresis and tandem-mass spectrometry. These analyses lead to the identification of 604 pod proteins and 965 seed proteins, including 263 proteins distinguishing the pod. The complete data set is publicly available at http://www.cbs.dtu.dk/cgi-bin/lotus/db.cgi , where spots in a reference map are linked to experimental data, such as matched peptides, quantification values, and gene accessions. Identified pod proteins represented enzymes from 85 different metabolic pathways, including storage globulins and a late embryogenesis abundant protein. In contrast to seed maturation, pod maturation was associated with decreasing total protein content, especially proteins involved in protein biosynthesis and photosynthesis. Proteins detected only in pods included three enzymes participating in the urea cycle and four in nitrogen and amino group metabolism, highlighting the importance of nitrogen metabolism during pod development. Additionally, five legume seed proteins previously unassigned in the glutamate metabolism pathway were identified.

U2 - 10.1021/pr100511u

DO - 10.1021/pr100511u

M3 - Journal article

C2 - 20831161

VL - 9

SP - 5715

EP - 5726

JO - Journal of Proteome Research

JF - Journal of Proteome Research

SN - 1535-3893

IS - 11

ER -