Protein Surface and Core Dynamics Show Concerted Hydration-Dependent Activation

Kathleen Wood; François-Xavier Gallat; Renee Otten; Auke J van Heel; Mathilde Lethier; Lambert van Eijck; Martine Moulin; Michael Haertlein; Martin Weik; Frans A.A. Mulder

doi:10.1002/anie.201205898

Protein Surface and Core Dynamics Show Concerted Hydration-Dependent Activation

Kathleen Wood, François-Xavier Gallat, Renee Otten, Auke J van Heel, Mathilde Lethier, Lambert van Eijck, Martine Moulin, Michael Haertlein, Martin Weik, Frans A.A. Mulder

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

30 Citations (Scopus)

Abstract

By specifically labeling leucine/valine methyl groups and lysine side chains "inside" and "outside" dynamics of proteins on the nanosecond timescale are compared using neutron scattering. Surprisingly, both groups display similar dynamics as a function of temperature, and the buried hydrophobic core is sensitive to hydration and undergoes a dynamical transition.

Original language	English
Journal	Angewandte Chemie International Edition
Volume	52
Issue	2
Pages (from-to)	665-668
Number of pages	4
ISSN	1433-7851
DOIs	https://doi.org/10.1002/anie.201205898
Publication status	Published - 2013

Keywords

NMR neutron scattering protein dynamics

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10.1002/anie.201205898

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Danish NMR Discussion Group Meeting
Mulder, F. A. A. (Participant)
21 Jan 2013 → 22 Jan 2013
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Cite this

@article{4a578dbd2cab4ef3b6b33844ae0c013c,

title = "Protein Surface and Core Dynamics Show Concerted Hydration-Dependent Activation",

abstract = "By specifically labeling leucine/valine methyl groups and lysine side chains {"}inside{"} and {"}outside{"} dynamics of proteins on the nanosecond timescale are compared using neutron scattering. Surprisingly, both groups display similar dynamics as a function of temperature, and the buried hydrophobic core is sensitive to hydration and undergoes a dynamical transition.",

keywords = "NMR neutron scattering protein dynamics",

author = "Kathleen Wood and Fran{\c c}ois-Xavier Gallat and Renee Otten and \{van Heel\}, \{Auke J\} and Mathilde Lethier and \{van Eijck\}, Lambert and Martine Moulin and Michael Haertlein and Martin Weik and Mulder, \{Frans A.A.\}",

note = "Copyright {\textcopyright} 2012 WILEY-VCH Verlag GmbH \& Co. KGaA, Weinheim.",

year = "2013",

doi = "10.1002/anie.201205898",

language = "English",

volume = "52",

pages = "665--668",

journal = "Angewandte Chemie International Edition",

issn = "1433-7851",

publisher = "John Wiley and Sons Ltd",

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}

Protein Surface and Core Dynamics Show Concerted Hydration-Dependent Activation. / Wood, Kathleen; Gallat, François-Xavier; Otten, Renee et al.
In: Angewandte Chemie International Edition, Vol. 52, No. 2, 2013, p. 665-668.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

TY - JOUR

T1 - Protein Surface and Core Dynamics Show Concerted Hydration-Dependent Activation

AU - Wood, Kathleen

AU - Gallat, François-Xavier

AU - Otten, Renee

AU - van Heel, Auke J

AU - Lethier, Mathilde

AU - van Eijck, Lambert

AU - Moulin, Martine

AU - Haertlein, Michael

AU - Weik, Martin

AU - Mulder, Frans A.A.

PY - 2013

Y1 - 2013

N2 - By specifically labeling leucine/valine methyl groups and lysine side chains "inside" and "outside" dynamics of proteins on the nanosecond timescale are compared using neutron scattering. Surprisingly, both groups display similar dynamics as a function of temperature, and the buried hydrophobic core is sensitive to hydration and undergoes a dynamical transition.

AB - By specifically labeling leucine/valine methyl groups and lysine side chains "inside" and "outside" dynamics of proteins on the nanosecond timescale are compared using neutron scattering. Surprisingly, both groups display similar dynamics as a function of temperature, and the buried hydrophobic core is sensitive to hydration and undergoes a dynamical transition.

KW - NMR neutron scattering protein dynamics

U2 - 10.1002/anie.201205898

DO - 10.1002/anie.201205898

M3 - Journal article

C2 - 23154872

SN - 1433-7851

VL - 52

SP - 665

EP - 668

JO - Angewandte Chemie International Edition

JF - Angewandte Chemie International Edition

IS - 2

ER -

Protein Surface and Core Dynamics Show Concerted Hydration-Dependent Activation

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