Protein adsorption at nanopatterned surfaces studied by QCM-D and SPR

Stine Kristensen; Gitte Albinus Pedersen; Lene Niemann Nejsum; Duncan S Sutherland

doi:10.1021/jp4038528

Protein adsorption at nanopatterned surfaces studied by QCM-D and SPR

Stine Kristensen, Gitte Albinus Pedersen, Lene Niemann Nejsum, Duncan S Sutherland

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

13 Citations (Scopus)

Abstract

This paper presents the use of the quartz microbalance with dissipation combined with surface plasmon resonance to probe protein adsorption at nanopatterned surfaces. Three different types of adsorbing materials, representing rigid discrete nanoparticles, dense protein films and soft low density films have been studied on systematic varied circular nanostructures in the 100 nm to 1000 nm size range. Analysis and quantification of the QCM-D response from larger nanostructures could be understood and quantified in the same way as for homogeneous surfaces, while that for nanostructures of 100 nm and 200 nm diameter was significantly underestimated. Our findings suggest a size limitation of those techniques in analysis of adsorption at nanofeatures.

Original language	English
Journal	Journal of Physical Chemistry Part B: Condensed Matter, Materials, Surfaces, Interfaces & Biophysical
Volume	117
Issue	36
Pages (from-to)	10376-10383
Number of pages	8
ISSN	1520-6106
DOIs	https://doi.org/10.1021/jp4038528
Publication status	Published - 12 Sept 2013

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title = "Protein adsorption at nanopatterned surfaces studied by QCM-D and SPR",

abstract = "This paper presents the use of the quartz microbalance with dissipation combined with surface plasmon resonance to probe protein adsorption at nanopatterned surfaces. Three different types of adsorbing materials, representing rigid discrete nanoparticles, dense protein films and soft low density films have been studied on systematic varied circular nanostructures in the 100 nm to 1000 nm size range. Analysis and quantification of the QCM-D response from larger nanostructures could be understood and quantified in the same way as for homogeneous surfaces, while that for nanostructures of 100 nm and 200 nm diameter was significantly underestimated. Our findings suggest a size limitation of those techniques in analysis of adsorption at nanofeatures. ",

author = "Stine Kristensen and Pedersen, {Gitte Albinus} and Nejsum, {Lene Niemann} and Sutherland, {Duncan S}",

year = "2013",

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doi = "10.1021/jp4038528",

language = "English",

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journal = "Journal of Physical Chemistry Part B: Condensed Matter, Materials, Surfaces, Interfaces & Biophysical",

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publisher = "American Chemical Society",

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Protein adsorption at nanopatterned surfaces studied by QCM-D and SPR. / Kristensen, Stine; Pedersen, Gitte Albinus; Nejsum, Lene Niemann et al.
In: Journal of Physical Chemistry Part B: Condensed Matter, Materials, Surfaces, Interfaces & Biophysical, Vol. 117, No. 36, 12.09.2013, p. 10376-10383.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

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T1 - Protein adsorption at nanopatterned surfaces studied by QCM-D and SPR

AU - Kristensen, Stine

AU - Pedersen, Gitte Albinus

AU - Nejsum, Lene Niemann

AU - Sutherland, Duncan S

PY - 2013/9/12

Y1 - 2013/9/12

N2 - This paper presents the use of the quartz microbalance with dissipation combined with surface plasmon resonance to probe protein adsorption at nanopatterned surfaces. Three different types of adsorbing materials, representing rigid discrete nanoparticles, dense protein films and soft low density films have been studied on systematic varied circular nanostructures in the 100 nm to 1000 nm size range. Analysis and quantification of the QCM-D response from larger nanostructures could be understood and quantified in the same way as for homogeneous surfaces, while that for nanostructures of 100 nm and 200 nm diameter was significantly underestimated. Our findings suggest a size limitation of those techniques in analysis of adsorption at nanofeatures.

AB - This paper presents the use of the quartz microbalance with dissipation combined with surface plasmon resonance to probe protein adsorption at nanopatterned surfaces. Three different types of adsorbing materials, representing rigid discrete nanoparticles, dense protein films and soft low density films have been studied on systematic varied circular nanostructures in the 100 nm to 1000 nm size range. Analysis and quantification of the QCM-D response from larger nanostructures could be understood and quantified in the same way as for homogeneous surfaces, while that for nanostructures of 100 nm and 200 nm diameter was significantly underestimated. Our findings suggest a size limitation of those techniques in analysis of adsorption at nanofeatures.

U2 - 10.1021/jp4038528

DO - 10.1021/jp4038528

M3 - Journal article

C2 - 23863103

SN - 1520-6106

VL - 117

SP - 10376

EP - 10383

JO - Journal of Physical Chemistry Part B: Condensed Matter, Materials, Surfaces, Interfaces & Biophysical

JF - Journal of Physical Chemistry Part B: Condensed Matter, Materials, Surfaces, Interfaces & Biophysical

IS - 36

ER -

Protein adsorption at nanopatterned surfaces studied by QCM-D and SPR

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