Prospective role of indigenous Exiguobacterium profundum PT2 in arsenic biotransformation and biosorption by planktonic cultures and biofilms

Saba Rehman, Rasmus Andreasen, Y li, Y Rehman, M. Ahmed, Rikke Louise Meyer, A.N. Sabri

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Abstract

Aims: The aim of this study was to analyse arsenic (As) transformation and biosorption by indigenous As-resistant bacteria both in planktonic and biofilm modes of growth. Methods and Results: As-resistant bacteria were isolated from industrial waste water and strain PT2, and identified as Exiguobacterium profundum through 16S rRNA gene sequencing was selected for further study. As transformation and biosorption by E. profundumPT2 was determined by HPLC-ICP-MS analysis. Planktonic cultures reduced 3·73 mmol l −1 As 5+ into As 3+ from artificial waste water effluent after 48-h incubation. In case of biosorption, planktonic cultures and biofilms exhibited 25·2 and 29·4 mg g −1 biomass biosorption, respectively. As biosorption kinetics followed Freundlich isotherm and pseudo second-order model. Biofilm formation peaked after 3 days of incubation, and in the presence of As stress, biofilm formation was significantly affected in contrast to control (P < 0·05). Homogeneous nature of mature biofilms with an increased demand of nutrients was revealed by minimum roughness and maximum surface to biovolume ratio measured through CLSM analysis. Conclusion: Indigenous As-resistant E. profundumPT2 was found capable of As transformation and biosorption both in the form of planktonic cultures and biofilms. Significance and Impact of the Study: Indigenous biofilm forming E. profundum PT2 revealing As biosorption and biotransformation potential is presented an eco-friendly and cost-effective source for As remediation that can be implemented for waste water treatment.

Original languageEnglish
JournalJournal of Applied Microbiology
Volume124
Issue2
Pages (from-to)431-443
Number of pages12
ISSN1364-5072
DOIs
Publication statusPublished - Feb 2018

Keywords

  • arsenic biotransformation
  • arsenic resistance
  • biofilms
  • bioremediation
  • wastewater treatment

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