Aarhus University Seal / Aarhus Universitets segl

Programmed dual-electrospun fibers with a 3D substrate-independent customized biomolecule gradient

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Standard

Programmed dual-electrospun fibers with a 3D substrate-independent customized biomolecule gradient. / Amagat, Jordi; Jørgensen, Mathias Lindh; Zhang, Zhongyang; Xu, Ruodan; Chen, Menglin.

In: Materials Today Communications, Vol. 26, 102066, 03.2021.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

APA

CBE

MLA

Vancouver

Author

Amagat, Jordi ; Jørgensen, Mathias Lindh ; Zhang, Zhongyang ; Xu, Ruodan ; Chen, Menglin. / Programmed dual-electrospun fibers with a 3D substrate-independent customized biomolecule gradient. In: Materials Today Communications. 2021 ; Vol. 26.

Bibtex

@article{c8971253ccc34078b51b50486d87174a,
title = "Programmed dual-electrospun fibers with a 3D substrate-independent customized biomolecule gradient",
abstract = "Chemotaxis has been found essential in many key biological processes, such as embryogenesis and tissue formation, cancer, wound healing, immunological disorders and inflammation etc. Spatial organization of biomolecules is, therefore, crucial in tissue engineering and disease modeling. Herein, we, for the first time, present a programmed dual-electrospun 3D scaffolds with customizable biomolecule gradients based on substrate-independent benzophenone (BP) photochemistry. Different customized fluorescent gradients/patterns were successfully obtained in polycaprolactone (PCL) fibers. In addition, a gradient of doxorubicin, a model anti-cancer drug, loaded by direct mixing was able to influence SW480 colorectal cancer cell viability locally. Further using a coagulation bath collector containing ethanol with low-surface tension, wet electrospun loosely packed PCL fibers were fabricated with a tailored gradient of PCL functionalized with BPITC (Benzophenone-4-isothicyanate), a heterobifunctional crosslinker, capable of inserting primary amine-binding sites into the PCL molecule/C–H bond by the substrate-independent photoreaction of BP. The so-called PCL-BPITC scaffolds could be conjugated with any target molecules (TM) with primary amines, such as proteins, and transform the PCL-BPITC gradient into a PCL-TM gradient. When a Horseradish Peroxidase (HRP)- antibody was used as a TM, a colorimetric assay similar to ELISA principle led to successful generation of the colorful product in the same gradient. Based on the substrate-independent BP photochemistry and possibility of other bifunctional crosslinkers, such as BP-maleimide, our findings suggest a great potential for the generation of 3D scaffolds of any electrospun fibers with mono/dual/multi customizable biochemical gradient(s), that may serve a new tool for broad chemotaxis-based biomedical applications such as tissue engineering and disease modeling.",
keywords = "Benzophenone, Customizable gradient, Programmed-electrospinning, Substrate-independent",
author = "Jordi Amagat and J{\o}rgensen, {Mathias Lindh} and Zhongyang Zhang and Ruodan Xu and Menglin Chen",
note = "Funding Information: We gratefully acknowledge Carlsberg Foundation ( CF19-0300 ), Aarhus University Research foundation ( AUFF-E-2015-FLS-7-27 ) for support of the research. Publisher Copyright: {\textcopyright} 2021 Elsevier Ltd Copyright: Copyright 2021 Elsevier B.V., All rights reserved.",
year = "2021",
month = mar,
doi = "10.1016/j.mtcomm.2021.102066",
language = "English",
volume = "26",
journal = "Materials Today Communications",
issn = "2352-4928",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Programmed dual-electrospun fibers with a 3D substrate-independent customized biomolecule gradient

AU - Amagat, Jordi

AU - Jørgensen, Mathias Lindh

AU - Zhang, Zhongyang

AU - Xu, Ruodan

AU - Chen, Menglin

N1 - Funding Information: We gratefully acknowledge Carlsberg Foundation ( CF19-0300 ), Aarhus University Research foundation ( AUFF-E-2015-FLS-7-27 ) for support of the research. Publisher Copyright: © 2021 Elsevier Ltd Copyright: Copyright 2021 Elsevier B.V., All rights reserved.

PY - 2021/3

Y1 - 2021/3

N2 - Chemotaxis has been found essential in many key biological processes, such as embryogenesis and tissue formation, cancer, wound healing, immunological disorders and inflammation etc. Spatial organization of biomolecules is, therefore, crucial in tissue engineering and disease modeling. Herein, we, for the first time, present a programmed dual-electrospun 3D scaffolds with customizable biomolecule gradients based on substrate-independent benzophenone (BP) photochemistry. Different customized fluorescent gradients/patterns were successfully obtained in polycaprolactone (PCL) fibers. In addition, a gradient of doxorubicin, a model anti-cancer drug, loaded by direct mixing was able to influence SW480 colorectal cancer cell viability locally. Further using a coagulation bath collector containing ethanol with low-surface tension, wet electrospun loosely packed PCL fibers were fabricated with a tailored gradient of PCL functionalized with BPITC (Benzophenone-4-isothicyanate), a heterobifunctional crosslinker, capable of inserting primary amine-binding sites into the PCL molecule/C–H bond by the substrate-independent photoreaction of BP. The so-called PCL-BPITC scaffolds could be conjugated with any target molecules (TM) with primary amines, such as proteins, and transform the PCL-BPITC gradient into a PCL-TM gradient. When a Horseradish Peroxidase (HRP)- antibody was used as a TM, a colorimetric assay similar to ELISA principle led to successful generation of the colorful product in the same gradient. Based on the substrate-independent BP photochemistry and possibility of other bifunctional crosslinkers, such as BP-maleimide, our findings suggest a great potential for the generation of 3D scaffolds of any electrospun fibers with mono/dual/multi customizable biochemical gradient(s), that may serve a new tool for broad chemotaxis-based biomedical applications such as tissue engineering and disease modeling.

AB - Chemotaxis has been found essential in many key biological processes, such as embryogenesis and tissue formation, cancer, wound healing, immunological disorders and inflammation etc. Spatial organization of biomolecules is, therefore, crucial in tissue engineering and disease modeling. Herein, we, for the first time, present a programmed dual-electrospun 3D scaffolds with customizable biomolecule gradients based on substrate-independent benzophenone (BP) photochemistry. Different customized fluorescent gradients/patterns were successfully obtained in polycaprolactone (PCL) fibers. In addition, a gradient of doxorubicin, a model anti-cancer drug, loaded by direct mixing was able to influence SW480 colorectal cancer cell viability locally. Further using a coagulation bath collector containing ethanol with low-surface tension, wet electrospun loosely packed PCL fibers were fabricated with a tailored gradient of PCL functionalized with BPITC (Benzophenone-4-isothicyanate), a heterobifunctional crosslinker, capable of inserting primary amine-binding sites into the PCL molecule/C–H bond by the substrate-independent photoreaction of BP. The so-called PCL-BPITC scaffolds could be conjugated with any target molecules (TM) with primary amines, such as proteins, and transform the PCL-BPITC gradient into a PCL-TM gradient. When a Horseradish Peroxidase (HRP)- antibody was used as a TM, a colorimetric assay similar to ELISA principle led to successful generation of the colorful product in the same gradient. Based on the substrate-independent BP photochemistry and possibility of other bifunctional crosslinkers, such as BP-maleimide, our findings suggest a great potential for the generation of 3D scaffolds of any electrospun fibers with mono/dual/multi customizable biochemical gradient(s), that may serve a new tool for broad chemotaxis-based biomedical applications such as tissue engineering and disease modeling.

KW - Benzophenone

KW - Customizable gradient

KW - Programmed-electrospinning

KW - Substrate-independent

UR - http://www.scopus.com/inward/record.url?scp=85099877970&partnerID=8YFLogxK

U2 - 10.1016/j.mtcomm.2021.102066

DO - 10.1016/j.mtcomm.2021.102066

M3 - Journal article

AN - SCOPUS:85099877970

VL - 26

JO - Materials Today Communications

JF - Materials Today Communications

SN - 2352-4928

M1 - 102066

ER -