Profiling of long non-coding RNAs identifies LINC00958 and LINC01296 as candidate oncogenes in bladder cancer

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Profiling of long non-coding RNAs identifies LINC00958 and LINC01296 as candidate oncogenes in bladder cancer. / Seitz, Anna Katharina; Christensen, Lise Lotte; Christensen, Emil; Faarkrog, Kasper; Ostenfeld, Marie Stampe; Hedegaard, Jakob; Nordentoft, Iver; Nielsen, Morten Muhlig; Palmfeldt, Johan; Thomson, Michelle; Jensen, Michael Theis Solgaard; Nawroth, Roman; Maurer, Tobias; Orntoft, Torben Falck; Jensen, Jorgen Bjerggaard; Damgaard, Christian Kroun; Dyrskjot, Lars.

In: Scientific Reports, Vol. 7, 395, 24.03.2017.

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@article{6237561e5efc443ab123a3d5c075d8c1,
title = "Profiling of long non-coding RNAs identifies LINC00958 and LINC01296 as candidate oncogenes in bladder cancer",
abstract = "Aberrant expression of long non-coding RNAs (lncRNAs) has been regarded as a critical component in bladder cancer (BC) and lncRNAs have been associated with BC development and progression although their overall expression and functional significance is still unclear. The aim of our study was to identify novel lncRNAs with a functional role in BC carcinogenesis. RNA-sequencing was used to identify aberrantly expressed lncRNAs in 8 normal and 72 BC samples. We identified 89 lncRNAs that were significantly dys-regulated in BC. Five lncRNAs; LINC00958, LINC01296, LINC00355, LNCCMC1-1 and LNC-ALX1-2 were selected for further analyses. Silencing of LINC00958 or LINC01296 in vitro reduced both cell viability and migration. Knock-down of LINC00958 also affected invasion and resistance to anoikis. These cellular effects could be linked to direct/indirect regulation of protein coding mRNAs involved in cell death/survival, proliferation and cellular movement. Finally, we showed that LINC00958 binds proteins involved in regulation and initiation of translation and in post-transcriptional modification of RNA, including Metadherin, which has previously been associated with BC. Our analyses identified novel lncRNAs in BC that likely act as oncogenic drivers contributing to an aggressive cancerous phenotype likely through interaction with proteins involved in initiation of translation and/or post-transcriptional modification of RNA.",
keywords = "UROTHELIAL CARCINOMA, TUMOR-SUPPRESSOR, UP-REGULATION, CELL-LINES, EXPRESSION, HOTAIR, METASTASIS, SIGNATURE, EVOLUTION, REVEALS",
author = "Seitz, {Anna Katharina} and Christensen, {Lise Lotte} and Emil Christensen and Kasper Faarkrog and Ostenfeld, {Marie Stampe} and Jakob Hedegaard and Iver Nordentoft and Nielsen, {Morten Muhlig} and Johan Palmfeldt and Michelle Thomson and Jensen, {Michael Theis Solgaard} and Roman Nawroth and Tobias Maurer and Orntoft, {Torben Falck} and Jensen, {Jorgen Bjerggaard} and Damgaard, {Christian Kroun} and Lars Dyrskjot",
year = "2017",
month = mar,
day = "24",
doi = "10.1038/s41598-017-00327-0",
language = "English",
volume = "7",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",

}

RIS

TY - JOUR

T1 - Profiling of long non-coding RNAs identifies LINC00958 and LINC01296 as candidate oncogenes in bladder cancer

AU - Seitz, Anna Katharina

AU - Christensen, Lise Lotte

AU - Christensen, Emil

AU - Faarkrog, Kasper

AU - Ostenfeld, Marie Stampe

AU - Hedegaard, Jakob

AU - Nordentoft, Iver

AU - Nielsen, Morten Muhlig

AU - Palmfeldt, Johan

AU - Thomson, Michelle

AU - Jensen, Michael Theis Solgaard

AU - Nawroth, Roman

AU - Maurer, Tobias

AU - Orntoft, Torben Falck

AU - Jensen, Jorgen Bjerggaard

AU - Damgaard, Christian Kroun

AU - Dyrskjot, Lars

PY - 2017/3/24

Y1 - 2017/3/24

N2 - Aberrant expression of long non-coding RNAs (lncRNAs) has been regarded as a critical component in bladder cancer (BC) and lncRNAs have been associated with BC development and progression although their overall expression and functional significance is still unclear. The aim of our study was to identify novel lncRNAs with a functional role in BC carcinogenesis. RNA-sequencing was used to identify aberrantly expressed lncRNAs in 8 normal and 72 BC samples. We identified 89 lncRNAs that were significantly dys-regulated in BC. Five lncRNAs; LINC00958, LINC01296, LINC00355, LNCCMC1-1 and LNC-ALX1-2 were selected for further analyses. Silencing of LINC00958 or LINC01296 in vitro reduced both cell viability and migration. Knock-down of LINC00958 also affected invasion and resistance to anoikis. These cellular effects could be linked to direct/indirect regulation of protein coding mRNAs involved in cell death/survival, proliferation and cellular movement. Finally, we showed that LINC00958 binds proteins involved in regulation and initiation of translation and in post-transcriptional modification of RNA, including Metadherin, which has previously been associated with BC. Our analyses identified novel lncRNAs in BC that likely act as oncogenic drivers contributing to an aggressive cancerous phenotype likely through interaction with proteins involved in initiation of translation and/or post-transcriptional modification of RNA.

AB - Aberrant expression of long non-coding RNAs (lncRNAs) has been regarded as a critical component in bladder cancer (BC) and lncRNAs have been associated with BC development and progression although their overall expression and functional significance is still unclear. The aim of our study was to identify novel lncRNAs with a functional role in BC carcinogenesis. RNA-sequencing was used to identify aberrantly expressed lncRNAs in 8 normal and 72 BC samples. We identified 89 lncRNAs that were significantly dys-regulated in BC. Five lncRNAs; LINC00958, LINC01296, LINC00355, LNCCMC1-1 and LNC-ALX1-2 were selected for further analyses. Silencing of LINC00958 or LINC01296 in vitro reduced both cell viability and migration. Knock-down of LINC00958 also affected invasion and resistance to anoikis. These cellular effects could be linked to direct/indirect regulation of protein coding mRNAs involved in cell death/survival, proliferation and cellular movement. Finally, we showed that LINC00958 binds proteins involved in regulation and initiation of translation and in post-transcriptional modification of RNA, including Metadherin, which has previously been associated with BC. Our analyses identified novel lncRNAs in BC that likely act as oncogenic drivers contributing to an aggressive cancerous phenotype likely through interaction with proteins involved in initiation of translation and/or post-transcriptional modification of RNA.

KW - UROTHELIAL CARCINOMA

KW - TUMOR-SUPPRESSOR

KW - UP-REGULATION

KW - CELL-LINES

KW - EXPRESSION

KW - HOTAIR

KW - METASTASIS

KW - SIGNATURE

KW - EVOLUTION

KW - REVEALS

U2 - 10.1038/s41598-017-00327-0

DO - 10.1038/s41598-017-00327-0

M3 - Journal article

C2 - 28341852

VL - 7

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 395

ER -