Probiotic Effects on Multispecies Biofilm Composition, Architecture, and Caries Activity In Vitro

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

  • Zhihui Chen, Charité-Universitätsmedizin, Berlin
  • ,
  • Sebastian Schlafer
  • Gerd Göstemeyer, Charité-Universitätsmedizin, Berlin
  • ,
  • Falk Schwendicke, Charité-Universitätsmedizin, Berlin

While probiotics have been tested for their anti-caries effect in vitro and also clinically, there is a lack of understanding of their effects on complex dental biofilms. We assessed two probiotics, Lactobacillus reuteri and Streptococcus oligofermentans, on a continuous-cultured model containing Streptococcus mutans, Lactobacillus rhamnosus and Actinomyces naeslundii. Cariogenic biofilms were grown on bovine enamel specimens and daily challenged with L. reuteri or S. oligofermentans whole culture (LC/SC) or cell-free supernatant (LS/SS) or medium only (negative control, NC) (n = 21/group) for 10 days. Biofilm was assessed via counting colony-forming units, quantitative polymerase chain reaction, and fluorescence in situ hybridization. Caries activity was determined by pH measurements and by assessing mineral loss (∆Z) using transverse microradiography. Both LC and SC significantly reduced total and strain-specific cariogenic bacterial numbers (p < 0.05). ∆Z was reduced in LC (mean ± SD: 1846.67 ± 317.89) and SC (3315.87 ± 617.30) compared to NC (4681.48 ± 495.18, p < 0.05). No significant reductions in bacterial numbers and ∆Z was induced by supernatants. Biofilm architecture was not considerably affected by probiotic applications. Viable probiotics L. reuteri and S. oligofermentans, but not their culture supernatants, could reduce the caries activity of multi-species biofilms in vitro.

Original languageEnglish
Article number1272
JournalMicroorganisms
Volume8
Issue9
Number of pages13
ISSN2076-2607
DOIs
Publication statusPublished - Sep 2020

    Research areas

  • Biofilm model, Demineralization, Dental caries, Enamel, Transverse microradiography

See relations at Aarhus University Citationformats

ID: 195491863