Department of Economics and Business Economics

Presence of an epigenetic signature of prenatal cigarette smoke exposure in childhood

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

  • Christine Ladd-Acosta
  • ,
  • Chang Shu, Denmark
  • Brian K Lee, Denmark
  • Nicole Gidaya, Denmark
  • Alison Singer, Denmark
  • Laura A Schieve
  • ,
  • Diana Schendel
  • Nicole Jones, Denmark
  • Julie L Daniels, Denmark
  • Gayle C Windham, Denmark
  • Craig J Newschaffer, Denmark
  • Lisa A Croen
  • ,
  • Andrew P Feinberg, Denmark
  • M Daniele Fallin, Denmark

Prenatal exposure to tobacco smoke has lifelong health consequences. Epigenetic signatures such as differences in DNA methylation (DNAm) may be a biomarker of exposure and, further, might have functional significance for how in utero tobacco exposure may influence disease risk. Differences in infant DNAm associated with maternal smoking during pregnancy have been identified. Here we assessed whether these infant DNAm patterns are detectible in early childhood, whether they are specific to smoking, and whether childhood DNAm can classify prenatal smoke exposure status. Using the Infinium 450K array, we measured methylation at 26 CpG loci that were previously associated with prenatal smoking in infant cord blood from 572 children, aged 3-5, with differing prenatal exposure to cigarette smoke in the Study to Explore Early Development (SEED). Striking concordance was found between the pattern of prenatal smoking associated DNAm among preschool aged children in SEED and those observed at birth in other studies. These DNAm changes appear to be tobacco-specific. Support vector machine classification models and 10-fold cross-validation were applied to show classification accuracy for childhood DNAm at these 26 sites as a biomarker of prenatal smoking exposure. Classification models showed prenatal exposure to smoking can be assigned with 81% accuracy using childhood DNAm patterns at these 26 loci. These findings support the potential for blood-derived DNAm measurements to serve as biomarkers for prenatal exposure.

Original languageEnglish
JournalEnvironmental Research
Volume144
IssuePart A
Pages (from-to)139-148
Number of pages10
ISSN0013-9351
DOIs
Publication statusPublished - 20 Nov 2015

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