Potassium depletion induces cellular conversion in the outer medullary collecting duct altering Notch signaling pathway

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

  • Anna Iervolino, Biogem S.c.a.r.l.
  • ,
  • Federica Prosperi, Biogem S.c.a.r.l.
  • ,
  • Luigi R. De La Motte, Biogem S.c.a.r.l.
  • ,
  • Federica Petrillo
  • Manuela Spagnuolo, Biogem S.c.a.r.l.
  • ,
  • Mariavittoria D’Acierno, Biogem S.c.a.r.l., Seconda Università degli Studi di Napoli
  • ,
  • Sabrina Siccardi, Biogem S.c.a.r.l., Seconda Università degli Studi di Napoli
  • ,
  • Alessandra F. Perna, Seconda Università degli Studi di Napoli
  • ,
  • Birgitte M. Christensen
  • Sebastian Frische
  • Giovambattista Capasso, Biogem S.c.a.r.l., Seconda Università degli Studi di Napoli
  • ,
  • Francesco Trepiccione, Biogem S.c.a.r.l., Department of Translational Medical Sciences, Seconda Università degli Studi di Napoli

Potassium depletion affects AQP2 expression and the cellular composition of the kidney collecting duct. This, in turn, contributes to the development of a secondary form of nephrogenic diabetes insipidus and hypokalemic nephropathy. Here we show that after 14 days of potassium depletion, the cellular fraction of A-type intercalated cells increases while the fraction of principal cells decreases along the outer medullary collecting duct in rats. The intercalated cells acquired a novel distribution pattern forming rows of cells attached to each other. These morphological changes occur progressively and reverse after 7 days of recovery on normal rat chow diet. The cellular remodeling mainly occurred in the inner stripe of outer medulla similar to the previously seen effect of lithium on the collecting duct cellular profile. The cellular remodeling is associated with the appearance of cells double labelled with both specific markers of principal and type-A intercalated cells. The appearance of this cell type was associated with the downregulation of the Notch signaling via the Hes1 pathways. These results show that the epithelium of the collecting duct has a high degree of plasticity and that Notch signaling likely plays a key role during hypokalemia.

Original languageEnglish
Article number5708
JournalScientific Reports
Volume10
Issue1
Number of pages10
ISSN2045-2322
DOIs
Publication statusPublished - 2020

See relations at Aarhus University Citationformats

ID: 184506144