Porcine synapsin 1: SYN1 gene analysis and functional characterization of the promoter

Claus Hedegaard; Kasper Kjaer-Sorensen; Lone Bruhn Madsen; Carina Henriksen; Jamal Momeni; Christian Bendixen; Claus Oxvig; Knud Erik Larsen

doi:10.1016/j.fob.2013.10.002

Porcine synapsin 1: SYN1 gene analysis and functional characterization of the promoter

Claus Hedegaard, Kasper Kjaer-Sorensen, Lone Bruhn Madsen, Carina Henriksen, Jamal Momeni, Christian Bendixen, Claus Oxvig, Knud Erik Larsen

Department of Biomedicine - Danish Biomembrane Research Centre

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

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Abstract

Synapsin 1 (SYN1) is a phosphoprotein involved in nerve signal transmission. The porcine SYN1 promoter orthologue was cloned and characterized to provide a means of expressing a transgene specifically in neurons. The nucleotide sequence of the promoter displayed a high degree of conservation of elements responsible for neuron-specific expression. Expression analysis of SYN1 demonstrated presence of transcript during embryonic development. Analysis of GFP expression in transgenic zebrafish embryos suggests that the pig SYN1 promoter directs expression in neuronal cells. Thus, the SYN1 promoter is a good candidate for use in the generation of pig models of human neurodegenerative disorders

Original language	English
Journal	F E B S Open Bio
Volume	3
Pages (from-to)	411–420
Number of pages	10
ISSN	2211-5463
DOIs	https://doi.org/10.1016/j.fob.2013.10.002
Publication status	Published - 2013

Keywords

GFP
Neuron-specific promoter
Pig
Transgenic
Zebrafish

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title = "Porcine synapsin 1: SYN1 gene analysis and functional characterization of the promoter",

abstract = "Synapsin 1 (SYN1) is a phosphoprotein involved in nerve signal transmission. The porcine SYN1 promoter orthologue was cloned and characterized to provide a means of expressing a transgene specifically in neurons. The nucleotide sequence of the promoter displayed a high degree of conservation of elements responsible for neuron-specific expression. Expression analysis of SYN1 demonstrated presence of transcript during embryonic development. Analysis of GFP expression in transgenic zebrafish embryos suggests that the pig SYN1 promoter directs expression in neuronal cells. Thus, the SYN1 promoter is a good candidate for use in the generation of pig models of human neurodegenerative disorders",

keywords = "GFP, Neuron-specific promoter, Pig, Transgenic, Zebrafish",

author = "Claus Hedegaard and Kasper Kjaer-Sorensen and Madsen, {Lone Bruhn} and Carina Henriksen and Jamal Momeni and Christian Bendixen and Claus Oxvig and Larsen, {Knud Erik}",

year = "2013",

doi = "10.1016/j.fob.2013.10.002",

language = "English",

volume = "3",

pages = "411–420",

journal = "F E B S Open Bio",

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T1 - Porcine synapsin 1: SYN1 gene analysis and functional characterization of the promoter

AU - Hedegaard, Claus

AU - Kjaer-Sorensen, Kasper

AU - Madsen, Lone Bruhn

AU - Henriksen, Carina

AU - Momeni, Jamal

AU - Bendixen, Christian

AU - Oxvig, Claus

AU - Larsen, Knud Erik

PY - 2013

Y1 - 2013

N2 - Synapsin 1 (SYN1) is a phosphoprotein involved in nerve signal transmission. The porcine SYN1 promoter orthologue was cloned and characterized to provide a means of expressing a transgene specifically in neurons. The nucleotide sequence of the promoter displayed a high degree of conservation of elements responsible for neuron-specific expression. Expression analysis of SYN1 demonstrated presence of transcript during embryonic development. Analysis of GFP expression in transgenic zebrafish embryos suggests that the pig SYN1 promoter directs expression in neuronal cells. Thus, the SYN1 promoter is a good candidate for use in the generation of pig models of human neurodegenerative disorders

AB - Synapsin 1 (SYN1) is a phosphoprotein involved in nerve signal transmission. The porcine SYN1 promoter orthologue was cloned and characterized to provide a means of expressing a transgene specifically in neurons. The nucleotide sequence of the promoter displayed a high degree of conservation of elements responsible for neuron-specific expression. Expression analysis of SYN1 demonstrated presence of transcript during embryonic development. Analysis of GFP expression in transgenic zebrafish embryos suggests that the pig SYN1 promoter directs expression in neuronal cells. Thus, the SYN1 promoter is a good candidate for use in the generation of pig models of human neurodegenerative disorders

KW - GFP

KW - Neuron-specific promoter

KW - Pig

KW - Transgenic

KW - Zebrafish

U2 - 10.1016/j.fob.2013.10.002

DO - 10.1016/j.fob.2013.10.002

M3 - Journal article

C2 - 24251104

SN - 2211-5463

VL - 3

SP - 411

EP - 420

JO - F E B S Open Bio

JF - F E B S Open Bio

ER -

Porcine synapsin 1: SYN1 gene analysis and functional characterization of the promoter

Abstract

Keywords

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