Poly(norepinephrine) as a functional bio-interface for neuronal differentiation on electrospun fibers

Mehmet Berat Taskin; Ruodan Xu; Huiling Zhao; Xueqin Wang; Mingdong Dong; Flemming Besenbacher; Menglin Chen

doi:10.1039/c5cp00413f

Poly(norepinephrine) as a functional bio-interface for neuronal differentiation on electrospun fibers

Mehmet Berat Taskin, Ruodan Xu, Huiling Zhao, Xueqin Wang, Mingdong Dong, Flemming Besenbacher, Menglin Chen

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

39 Citations (Scopus)

Abstract

Based on the catecholic chemistry of a mussel inspired coating, norepinephrine (NE), a catecholamine found both in neurotransmitters and mussel adhesive proteins, was for the first time applied as a unique bio-interface integrating multi-functions facilitating PC12 neuron-like differentiation. A uniform, ultra-smooth pNE coating was achieved on electrospun submicron PLCL fibers, proven by surface characterization. The introduced catechol groups from pNE were further used to not only anchor collagen to enhance cell adhesion but also localize nerve growth factor to promote neuron-like differentiation. The obtained pNE-collagen coating was found to be a superior substrate for PC12 differentiation, confirmed by both cellular toxicity/viability assays and immunochemical staining. The aligned PLCL fiber conformation further steered neurite formation along the fiber direction and contributed to neurite extension and increased the number of neurites per cell body. This facile pNE coating might lead to a more efficient use of growth factor, drugs and other bioactive molecules with lower loading dosage and sustained activity resulting in enhanced therapeutic effects and decreased adverse effects.

Original language	English
Journal	Physical Chemistry Chemical Physics
Volume	17
Issue	14
Pages (from-to)	9446-53
Number of pages	8
ISSN	1463-9076
DOIs	https://doi.org/10.1039/c5cp00413f
Publication status	Published - 14 Apr 2015

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title = "Poly(norepinephrine) as a functional bio-interface for neuronal differentiation on electrospun fibers",

abstract = "Based on the catecholic chemistry of a mussel inspired coating, norepinephrine (NE), a catecholamine found both in neurotransmitters and mussel adhesive proteins, was for the first time applied as a unique bio-interface integrating multi-functions facilitating PC12 neuron-like differentiation. A uniform, ultra-smooth pNE coating was achieved on electrospun submicron PLCL fibers, proven by surface characterization. The introduced catechol groups from pNE were further used to not only anchor collagen to enhance cell adhesion but also localize nerve growth factor to promote neuron-like differentiation. The obtained pNE-collagen coating was found to be a superior substrate for PC12 differentiation, confirmed by both cellular toxicity/viability assays and immunochemical staining. The aligned PLCL fiber conformation further steered neurite formation along the fiber direction and contributed to neurite extension and increased the number of neurites per cell body. This facile pNE coating might lead to a more efficient use of growth factor, drugs and other bioactive molecules with lower loading dosage and sustained activity resulting in enhanced therapeutic effects and decreased adverse effects.",

author = "Taskin, {Mehmet Berat} and Ruodan Xu and Huiling Zhao and Xueqin Wang and Mingdong Dong and Flemming Besenbacher and Menglin Chen",

year = "2015",

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doi = "10.1039/c5cp00413f",

language = "English",

volume = "17",

pages = "9446--53",

journal = "Physical Chemistry Chemical Physics",

issn = "1463-9076",

publisher = "Royal Society of Chemistry",

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Poly(norepinephrine) as a functional bio-interface for neuronal differentiation on electrospun fibers. / Taskin, Mehmet Berat; Xu, Ruodan; Zhao, Huiling et al.
In: Physical Chemistry Chemical Physics, Vol. 17, No. 14, 14.04.2015, p. 9446-53.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

TY - JOUR

T1 - Poly(norepinephrine) as a functional bio-interface for neuronal differentiation on electrospun fibers

AU - Taskin, Mehmet Berat

AU - Xu, Ruodan

AU - Zhao, Huiling

AU - Wang, Xueqin

AU - Dong, Mingdong

AU - Besenbacher, Flemming

AU - Chen, Menglin

PY - 2015/4/14

Y1 - 2015/4/14

N2 - Based on the catecholic chemistry of a mussel inspired coating, norepinephrine (NE), a catecholamine found both in neurotransmitters and mussel adhesive proteins, was for the first time applied as a unique bio-interface integrating multi-functions facilitating PC12 neuron-like differentiation. A uniform, ultra-smooth pNE coating was achieved on electrospun submicron PLCL fibers, proven by surface characterization. The introduced catechol groups from pNE were further used to not only anchor collagen to enhance cell adhesion but also localize nerve growth factor to promote neuron-like differentiation. The obtained pNE-collagen coating was found to be a superior substrate for PC12 differentiation, confirmed by both cellular toxicity/viability assays and immunochemical staining. The aligned PLCL fiber conformation further steered neurite formation along the fiber direction and contributed to neurite extension and increased the number of neurites per cell body. This facile pNE coating might lead to a more efficient use of growth factor, drugs and other bioactive molecules with lower loading dosage and sustained activity resulting in enhanced therapeutic effects and decreased adverse effects.

AB - Based on the catecholic chemistry of a mussel inspired coating, norepinephrine (NE), a catecholamine found both in neurotransmitters and mussel adhesive proteins, was for the first time applied as a unique bio-interface integrating multi-functions facilitating PC12 neuron-like differentiation. A uniform, ultra-smooth pNE coating was achieved on electrospun submicron PLCL fibers, proven by surface characterization. The introduced catechol groups from pNE were further used to not only anchor collagen to enhance cell adhesion but also localize nerve growth factor to promote neuron-like differentiation. The obtained pNE-collagen coating was found to be a superior substrate for PC12 differentiation, confirmed by both cellular toxicity/viability assays and immunochemical staining. The aligned PLCL fiber conformation further steered neurite formation along the fiber direction and contributed to neurite extension and increased the number of neurites per cell body. This facile pNE coating might lead to a more efficient use of growth factor, drugs and other bioactive molecules with lower loading dosage and sustained activity resulting in enhanced therapeutic effects and decreased adverse effects.

U2 - 10.1039/c5cp00413f

DO - 10.1039/c5cp00413f

M3 - Journal article

C2 - 25766518

SN - 1463-9076

VL - 17

SP - 9446

EP - 9453

JO - Physical Chemistry Chemical Physics

JF - Physical Chemistry Chemical Physics

IS - 14

ER -

Poly(norepinephrine) as a functional bio-interface for neuronal differentiation on electrospun fibers

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