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Polarized Trafficking of the Sorting Receptor SorLA in Neurons and MDCK Cells

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The sorting receptor SorLA is highly expressed in neurons and is also found in other polarized cells. The receptor has been reported to participate in the trafficking of several ligands, some of which are linked to human diseases, including the amyloid precursor protein, TrkB and lipoprotein lipase (LpL). Despite this, only the trafficking in non-polarized cells has been described so far. Due to the many differences between polarized and non-polarized cells, we examined the localization and trafficking of SorLA in epithelial Madin-Darby canine kidney (MDCK) cells and rat hippocampal neurons. We show that SorLA is mainly found in sorting endosomes and on the basolateral surface of MDCK cells and in the somatodendritic domain of neurons. This polarized distribution of SorLA respectivly depends on an acidic cluster and an extended version of this cluster and involves the cellular adaptor complex AP-1. Furthermore, we show that SorLA can mediate transcytosis across a tight cell layer. This article is protected by copyright. All rights reserved.

Original languageEnglish
JournalF E B S Journal
Pages (from-to)2476–2493
Publication statusPublished - 18 May 2016

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