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Podosome formation and development in monocytes restricted by the nanoscale spatial distribution of ICAM1

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  • Andreas Søe Andersen
  • ,
  • Hüsnü Aslan, Denmark
  • Mingdong Dong
  • Xingyu Jiang, National Center for Nanoscience and Technology (NCNST), Beijing, China
  • Duncan Sutherland

We studied podosome formation and development in activated monocytes (THP1) at ICAM1 (intercellular adhesion molecule 1) nanopatterns of circular and ring-shaped domains and show that cellular binding to a preclustered ICAM1 nanopattern requires ligand patches of at least 200 nm (corresponding to 14 or more integrins). Podosome-like adhesion formation depends on the structure of the ligand pattern under the developing podosome with larger single domains promoting adhesion in a single patch and multiple smaller domains allowing podosome formation by integration of at least 2 smaller domains on either side of the podosome core. Maturation to rosette structures and recruitment of proteases were only observed with macroscopic ICAM1 presentation.

Original languageEnglish
JournalNano Letters
Pages (from-to)2114-2121
Number of pages8
Publication statusPublished - 10 Feb 2016

    Research areas

  • ICAM1, THP1, adhesion, nanopattern, podosome

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