Podosome formation and development in monocytes restricted by the nanoscale spatial distribution of ICAM1

Andreas Søe Andersen; Hüsnü Aslan; Mingdong Dong; Xingyu Jiang; Duncan Sutherland

doi:10.1021/acs.nanolett.6b00519

Podosome formation and development in monocytes restricted by the nanoscale spatial distribution of ICAM1

Andreas Søe Andersen, Hüsnü Aslan, Mingdong Dong, Xingyu Jiang, Duncan Sutherland

Interdisciplinary Nanoscience Center - INANO-Fysik, iNANO-huset

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Letter › peer-review

13 Citations (Scopus)

Abstract

We studied podosome formation and development in activated monocytes (THP1) at ICAM1 (intercellular adhesion molecule 1) nanopatterns of circular and ring-shaped domains and show that cellular binding to a preclustered ICAM1 nanopattern requires ligand patches of at least 200 nm (corresponding to 14 or more integrins). Podosome-like adhesion formation depends on the structure of the ligand pattern under the developing podosome with larger single domains promoting adhesion in a single patch and multiple smaller domains allowing podosome formation by integration of at least 2 smaller domains on either side of the podosome core. Maturation to rosette structures and recruitment of proteases were only observed with macroscopic ICAM1 presentation.

Original language	English
Journal	Nano Letters
Volume	16
Issue	3
Pages (from-to)	2114-2121
Number of pages	8
ISSN	1530-6984
DOIs	https://doi.org/10.1021/acs.nanolett.6b00519
Publication status	Published - 9 Mar 2016

Keywords

ICAM1
THP1
adhesion
nanopattern
podosome

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10.1021/acs.nanolett.6b00519

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title = "Podosome formation and development in monocytes restricted by the nanoscale spatial distribution of ICAM1",

abstract = "We studied podosome formation and development in activated monocytes (THP1) at ICAM1 (intercellular adhesion molecule 1) nanopatterns of circular and ring-shaped domains and show that cellular binding to a preclustered ICAM1 nanopattern requires ligand patches of at least 200 nm (corresponding to 14 or more integrins). Podosome-like adhesion formation depends on the structure of the ligand pattern under the developing podosome with larger single domains promoting adhesion in a single patch and multiple smaller domains allowing podosome formation by integration of at least 2 smaller domains on either side of the podosome core. Maturation to rosette structures and recruitment of proteases were only observed with macroscopic ICAM1 presentation.",

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T1 - Podosome formation and development in monocytes restricted by the nanoscale spatial distribution of ICAM1

AU - Andersen, Andreas Søe

AU - Aslan, Hüsnü

AU - Dong, Mingdong

AU - Jiang, Xingyu

AU - Sutherland, Duncan

PY - 2016/3/9

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N2 - We studied podosome formation and development in activated monocytes (THP1) at ICAM1 (intercellular adhesion molecule 1) nanopatterns of circular and ring-shaped domains and show that cellular binding to a preclustered ICAM1 nanopattern requires ligand patches of at least 200 nm (corresponding to 14 or more integrins). Podosome-like adhesion formation depends on the structure of the ligand pattern under the developing podosome with larger single domains promoting adhesion in a single patch and multiple smaller domains allowing podosome formation by integration of at least 2 smaller domains on either side of the podosome core. Maturation to rosette structures and recruitment of proteases were only observed with macroscopic ICAM1 presentation.

AB - We studied podosome formation and development in activated monocytes (THP1) at ICAM1 (intercellular adhesion molecule 1) nanopatterns of circular and ring-shaped domains and show that cellular binding to a preclustered ICAM1 nanopattern requires ligand patches of at least 200 nm (corresponding to 14 or more integrins). Podosome-like adhesion formation depends on the structure of the ligand pattern under the developing podosome with larger single domains promoting adhesion in a single patch and multiple smaller domains allowing podosome formation by integration of at least 2 smaller domains on either side of the podosome core. Maturation to rosette structures and recruitment of proteases were only observed with macroscopic ICAM1 presentation.

KW - ICAM1

KW - THP1

KW - adhesion

KW - nanopattern

KW - podosome

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DO - 10.1021/acs.nanolett.6b00519

M3 - Letter

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SN - 1530-6984

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SP - 2114

EP - 2121

JO - Nano Letters

JF - Nano Letters

IS - 3

ER -

Podosome formation and development in monocytes restricted by the nanoscale spatial distribution of ICAM1

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Keywords

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