Plasma levels of H- and L-ficolin are increased in axial spondyloarthritis: Improvement of disease identification

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Plasma levels of H- and L-ficolin are increased in axial spondyloarthritis : Improvement of disease identification. / Troldborg, Anne; Thiel, Steffen; Elbaek, Clara Mistegaard; Hansen, Annette; Korsholm, Trine-Line; Stengaard-Pedersen, Kristian; Loft, Anne Gitte.

In: Clinical and Experimental Immunology, Vol. 199, No. 1, 01.2020, p. 79-87.

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@article{2c7aafe5c77e49098d3fa90854cce1dc,
title = "Plasma levels of H- and L-ficolin are increased in axial spondyloarthritis: Improvement of disease identification",
abstract = "Axial spondyloarthritis (axSpA) is a chronic inflammatory disease that primarily affects the axial skeleton. A predominance of innate versus adaptive immune responses have been reported in axSpA indicating a prominent autoinflammatory component of the disease. Little is known about the lectin pathway proteins (LPPs) of the complement system in relation to axSpA. We have investigated LPPs in patients with axSpA and control individuals. Plasma samples were obtained from a cross-sectional cohort of 120 patients with a clinical diagnosis of axSpA, and from 144 age and gender matched controls. The plasma concentrations of 11 LPPs were measured, using sandwich-type time-resolved immunoflourometric assays, in patients and controls, and related to clinical diagnosis and disease activity. Three LPPs (H-ficolin (ficolin-3), L-ficolin (ficolin-2) and collectin liver 1 (CL-L1)) were significantly higher in axSpA patients than in controls (p < 0.0001), and one LPP, collectin kidney 1 (CL-K1), was significantly lower (p < 0.0001). Further, combining H- or L-ficolin concentrations above the 75th -percentile of the respective H- or L-ficolin concentration measured in controls with HLA-B27 positivity yielded axSpA diagnostic specificities of 99% / 99% and positive likelihood ratios of 68 / 62, respectively. H-ficolin and L-ficolin plasma concentrations were found to be elevated in axSpA patients regardless of time since diagnosis. H-ficolin and L-ficolin may represent diagnostic biomarkers for patients with axSpA and should be further evaluated. Our results showed no association between disease activity and the measured LPP concentrations. This result might be due to the cross-sectional design, and should be further investigated.",
keywords = "axial spondyloarthritis, complement pathway, ficolins, innate immunity, lectin proteins, DIAGNOSIS, AUTOIMMUNE, COMPLEMENT, LECTIN PATHWAY, CLASSIFICATION, ANKYLOSING-SPONDYLITIS, PROTEINS",
author = "Anne Troldborg and Steffen Thiel and Elbaek, {Clara Mistegaard} and Annette Hansen and Trine-Line Korsholm and Kristian Stengaard-Pedersen and Loft, {Anne Gitte}",
note = "{\textcopyright} 2019 British Society for Immunology.",
year = "2020",
month = jan,
doi = "10.1111/cei.13374",
language = "English",
volume = "199",
pages = "79--87",
journal = "Clinical and Experimental Immunology",
issn = "0009-9104",
publisher = "Wiley-Blackwell Publishing Ltd.",
number = "1",

}

RIS

TY - JOUR

T1 - Plasma levels of H- and L-ficolin are increased in axial spondyloarthritis

T2 - Improvement of disease identification

AU - Troldborg, Anne

AU - Thiel, Steffen

AU - Elbaek, Clara Mistegaard

AU - Hansen, Annette

AU - Korsholm, Trine-Line

AU - Stengaard-Pedersen, Kristian

AU - Loft, Anne Gitte

N1 - © 2019 British Society for Immunology.

PY - 2020/1

Y1 - 2020/1

N2 - Axial spondyloarthritis (axSpA) is a chronic inflammatory disease that primarily affects the axial skeleton. A predominance of innate versus adaptive immune responses have been reported in axSpA indicating a prominent autoinflammatory component of the disease. Little is known about the lectin pathway proteins (LPPs) of the complement system in relation to axSpA. We have investigated LPPs in patients with axSpA and control individuals. Plasma samples were obtained from a cross-sectional cohort of 120 patients with a clinical diagnosis of axSpA, and from 144 age and gender matched controls. The plasma concentrations of 11 LPPs were measured, using sandwich-type time-resolved immunoflourometric assays, in patients and controls, and related to clinical diagnosis and disease activity. Three LPPs (H-ficolin (ficolin-3), L-ficolin (ficolin-2) and collectin liver 1 (CL-L1)) were significantly higher in axSpA patients than in controls (p < 0.0001), and one LPP, collectin kidney 1 (CL-K1), was significantly lower (p < 0.0001). Further, combining H- or L-ficolin concentrations above the 75th -percentile of the respective H- or L-ficolin concentration measured in controls with HLA-B27 positivity yielded axSpA diagnostic specificities of 99% / 99% and positive likelihood ratios of 68 / 62, respectively. H-ficolin and L-ficolin plasma concentrations were found to be elevated in axSpA patients regardless of time since diagnosis. H-ficolin and L-ficolin may represent diagnostic biomarkers for patients with axSpA and should be further evaluated. Our results showed no association between disease activity and the measured LPP concentrations. This result might be due to the cross-sectional design, and should be further investigated.

AB - Axial spondyloarthritis (axSpA) is a chronic inflammatory disease that primarily affects the axial skeleton. A predominance of innate versus adaptive immune responses have been reported in axSpA indicating a prominent autoinflammatory component of the disease. Little is known about the lectin pathway proteins (LPPs) of the complement system in relation to axSpA. We have investigated LPPs in patients with axSpA and control individuals. Plasma samples were obtained from a cross-sectional cohort of 120 patients with a clinical diagnosis of axSpA, and from 144 age and gender matched controls. The plasma concentrations of 11 LPPs were measured, using sandwich-type time-resolved immunoflourometric assays, in patients and controls, and related to clinical diagnosis and disease activity. Three LPPs (H-ficolin (ficolin-3), L-ficolin (ficolin-2) and collectin liver 1 (CL-L1)) were significantly higher in axSpA patients than in controls (p < 0.0001), and one LPP, collectin kidney 1 (CL-K1), was significantly lower (p < 0.0001). Further, combining H- or L-ficolin concentrations above the 75th -percentile of the respective H- or L-ficolin concentration measured in controls with HLA-B27 positivity yielded axSpA diagnostic specificities of 99% / 99% and positive likelihood ratios of 68 / 62, respectively. H-ficolin and L-ficolin plasma concentrations were found to be elevated in axSpA patients regardless of time since diagnosis. H-ficolin and L-ficolin may represent diagnostic biomarkers for patients with axSpA and should be further evaluated. Our results showed no association between disease activity and the measured LPP concentrations. This result might be due to the cross-sectional design, and should be further investigated.

KW - axial spondyloarthritis

KW - complement pathway

KW - ficolins

KW - innate immunity

KW - lectin proteins

KW - DIAGNOSIS

KW - AUTOIMMUNE

KW - COMPLEMENT

KW - LECTIN PATHWAY

KW - CLASSIFICATION

KW - ANKYLOSING-SPONDYLITIS

KW - PROTEINS

UR - http://www.scopus.com/inward/record.url?scp=85073932260&partnerID=8YFLogxK

U2 - 10.1111/cei.13374

DO - 10.1111/cei.13374

M3 - Journal article

C2 - 31518441

VL - 199

SP - 79

EP - 87

JO - Clinical and Experimental Immunology

JF - Clinical and Experimental Immunology

SN - 0009-9104

IS - 1

ER -