Plant-dairy protein blends: gelation behaviour in a filled particle matrix

Katherine Findlay Grasberger, Sandra Beyer Gregersen, Hanne Bak Jensen, Kristian Wejse Sanggaard, Milena Corredig

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The objective of this work was to study the structure formation in mixed protein systems containing oil droplets. A model system was prepared with skim milk, whey proteins and plant proteins to a final concentration of 8% (w/w) and homogenized with 20% (w/w) coconut fat. Three plant-derived protein sources (namely, pea isolate, oat concentrate, and lupin isolate) were added to skim milk at two different ratios of plant/dairy proteins. A blend containing only dairy protein was also used as reference, prepared with skim milk and whey protein isolate. The formation of structure in the emulsion filled gels after homogenization and heating were evaluated, using SDS-PAGE, light scattering, rheology and confocal microscopy. All emulsions showed a high viscosity due to the high extent of bridging flocculation amongst the oil droplets confirmed by a decrease in the particle size of emulsions upon the addition of SDS. After heating and cooling, the emulsions showed no macroscopic phase separation. The results demonstrated that structuring of complex gels can be modulated by controlling the composition at the oil-water interface, as well as by modulating processing conditions, capitalizing on the gelling properties of the single components in the matrix.

Original languageEnglish
Article number100198
JournalFood Structure
Publication statusPublished - Jul 2021


  • Emulsion filled gels
  • Lupin protein
  • Oat protein
  • Pea protein
  • Whey protein


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