piggyPrime: High-Efficacy Prime Editing in Human Cells Using piggyBac-Based DNA Transposition

Jonas Holst Wolff; Jakob Haldrup; Emil Aagaard Thomsen; Sofie Andersen; Jacob Giehm Mikkelsen

doi:10.3389/fgeed.2021.786893

piggyPrime: High-Efficacy Prime Editing in Human Cells Using piggyBac-Based DNA Transposition

Jonas Holst Wolff, Jakob Haldrup, Emil Aagaard Thomsen, Sofie Andersen, Jacob Giehm Mikkelsen^*

^*Corresponding author for this work

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

Abstract

Prime editing is a novel genome editing technology that allows a wide range of tailored genomic alterations. Prime editing does not involve homologous recombination, but suffers from low efficacy. Here, we demonstrate piggyPrime, a transfected single-vector system based on piggyBac DNA transposition for genomic integration of all prime editing components in human cells allowing easy and effective transgenesis with prime editing efficacies up to 100% in cell lines.

Original language	English
Article number	786893
Journal	Frontiers in Genome Editing
Volume	3
ISSN	2673-3439
DOIs	https://doi.org/10.3389/fgeed.2021.786893
Publication status	Published - 2021

Keywords

CRISPR
DNA transposition
pegRNA
piggyBac
prime editing
prime editing guide RNA

Access to Document

10.3389/fgeed.2021.786893

Cite this

@article{6c7bbb03d3a14c83b1ca49a22e055f03,

title = "piggyPrime: High-Efficacy Prime Editing in Human Cells Using piggyBac-Based DNA Transposition",

abstract = "Prime editing is a novel genome editing technology that allows a wide range of tailored genomic alterations. Prime editing does not involve homologous recombination, but suffers from low efficacy. Here, we demonstrate piggyPrime, a transfected single-vector system based on piggyBac DNA transposition for genomic integration of all prime editing components in human cells allowing easy and effective transgenesis with prime editing efficacies up to 100% in cell lines.",

keywords = "CRISPR, DNA transposition, pegRNA, piggyBac, prime editing, prime editing guide RNA",

author = "Wolff, {Jonas Holst} and Jakob Haldrup and Thomsen, {Emil Aagaard} and Sofie Andersen and Mikkelsen, {Jacob Giehm}",

note = "Publisher Copyright: Copyright {\textcopyright} 2021 Wolff, Haldrup, Thomsen, Andersen and Mikkelsen.",

year = "2021",

doi = "10.3389/fgeed.2021.786893",

language = "English",

volume = "3",

journal = "Frontiers in Genome Editing",

issn = "2673-3439",

publisher = "Frontiers Media S.A.",

}

TY - JOUR

T1 - piggyPrime

T2 - High-Efficacy Prime Editing in Human Cells Using piggyBac-Based DNA Transposition

AU - Wolff, Jonas Holst

AU - Haldrup, Jakob

AU - Thomsen, Emil Aagaard

AU - Andersen, Sofie

AU - Mikkelsen, Jacob Giehm

PY - 2021

Y1 - 2021

N2 - Prime editing is a novel genome editing technology that allows a wide range of tailored genomic alterations. Prime editing does not involve homologous recombination, but suffers from low efficacy. Here, we demonstrate piggyPrime, a transfected single-vector system based on piggyBac DNA transposition for genomic integration of all prime editing components in human cells allowing easy and effective transgenesis with prime editing efficacies up to 100% in cell lines.

AB - Prime editing is a novel genome editing technology that allows a wide range of tailored genomic alterations. Prime editing does not involve homologous recombination, but suffers from low efficacy. Here, we demonstrate piggyPrime, a transfected single-vector system based on piggyBac DNA transposition for genomic integration of all prime editing components in human cells allowing easy and effective transgenesis with prime editing efficacies up to 100% in cell lines.

KW - CRISPR

KW - DNA transposition

KW - pegRNA

KW - piggyBac

KW - prime editing

KW - prime editing guide RNA

UR - http://www.scopus.com/inward/record.url?scp=85137225670&partnerID=8YFLogxK

U2 - 10.3389/fgeed.2021.786893

DO - 10.3389/fgeed.2021.786893

M3 - Journal article

AN - SCOPUS:85137225670

SN - 2673-3439

VL - 3

JO - Frontiers in Genome Editing

JF - Frontiers in Genome Editing

M1 - 786893

ER -

piggyPrime: High-Efficacy Prime Editing in Human Cells Using piggyBac-Based DNA Transposition

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this