Photoluminescent Carbon Quantum Dot/Poly-L-Lysine Core-Shell Nanoparticles: A Novel Candidate for Gene Delivery

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  • Akbar Hasanzadeh, Iran University of Medical Sciences
  • ,
  • Mirza Ali Mofazzal Jahromi, Jahrom University of Medical Sciences
  • ,
  • Amir Abdoli, Jahrom University of Medical Science
  • ,
  • Hossein Mohammad-Beigi
  • ,
  • Yousef Fatahi, Tehran University of Medical Sciences
  • ,
  • Helena Nourizadeh, Iran University of Medical Sciences
  • ,
  • Hossein Zare, Iran University of Medical Sciences, Iran University of Science and Technology
  • ,
  • Jafar Kiani, Iran University of Medical Sciences
  • ,
  • Fatemeh Radmanesh, Shahid Beheshti University of Medical Sciences, Royan Institute for Stem Cell Biology and Technology
  • ,
  • Navid Rabiee, Shahid Beheshti University
  • ,
  • Mehdi Jahani, Sharif University of Technology
  • ,
  • Reza Mombeiny, Iran University of Medical Sciences
  • ,
  • Mahdi Karimi, Iran University of Medical Sciences

Cationic polymers such as poly-L-lysine (PLL) are able to interact electrostatically with DNA to produce polymeric systems with nanometric diameters due to the neutralization and accumulation of DNA. This study integrates the outstanding properties of carbon quantum dots (CQDs) with PLL to develop a novel gene delivery vehicle with a core-shell hybrid nanostructure. The CQD/PLL core-shell nanoparticles (NPs) were, therefore, synthesized in such a way that they had narrow size distribution and an average diameter under 10 nm, both of which were confirmed by dynamic light scattering (DLS) and transmission electron microscopy (TEM). Fourier transform infrared (FTIR) spectroscopy exhibited that the PLL passivation agents were formed on the CQDs through releasing amine groups on their surface. The positive charge of the CQD/PLL core-shell NPs reduced from +15 to nearly zero mV after being loaded with DNA at the weight ratio of 2:1. These traceable, water-soluble, biocompatible, and tunable photoluminescent NPs demonstrated a quantum yield of around 12% and a cellular uptake of nearly 70%. The NPs also showed no considerable toxicity to the human embryonic kidney (HEK)-293T cells. Hence, these novel CQD/PLL core-shell NPs hold great promise as a non-toxic and efficient gene delivery vector.

Original languageEnglish
Article number102118
JournalJournal of Drug Delivery Science and Technology
Volume61
Number of pages38
ISSN1773-2247
DOIs
Publication statusPublished - Feb 2021

    Research areas

  • Carbon quantum dot, Gene delivery, Nanoparticles, Poly-L-lysine

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