Phagophore closure, autophagosome maturation and autophagosome fusion during macroautophagy in the yeast Saccharomyces cerevisiae

TY - JOUR

T1 - Phagophore closure, autophagosome maturation and autophagosome fusion during macroautophagy in the yeast Saccharomyces cerevisiae

AU - Kraft, Claudine

AU - Reggiori, Fulvio

N1 - This article is protected by copyright. All rights reserved.

PY - 2024/1

Y1 - 2024/1

N2 - Macroautophagy, hereafter referred to as autophagy, is a complex process in which multiple membrane-remodeling events lead to the formation of a cisterna known as the phagophore, which then expands and closes into a double-membrane vesicle termed the autophagosome. During the past decade, enormous progress has been made in understanding the molecular function of the autophagy-related proteins and their role in generating these phagophores. In this Review, we discuss the current understanding of three membrane remodeling steps in autophagy that remain to be largely characterized; namely, the closure of phagophores, the maturation of the resulting autophagosomes into fusion-competent vesicles, and their fusion with vacuoles/lysosomes. Our review will mainly focus on the yeast Saccharomyces cerevisiae, which has been the leading model system for the study of molecular events in autophagy and has led to the discovery of the major mechanistic concepts, which have been found to be mostly conserved in higher eukaryotes.

AB - Macroautophagy, hereafter referred to as autophagy, is a complex process in which multiple membrane-remodeling events lead to the formation of a cisterna known as the phagophore, which then expands and closes into a double-membrane vesicle termed the autophagosome. During the past decade, enormous progress has been made in understanding the molecular function of the autophagy-related proteins and their role in generating these phagophores. In this Review, we discuss the current understanding of three membrane remodeling steps in autophagy that remain to be largely characterized; namely, the closure of phagophores, the maturation of the resulting autophagosomes into fusion-competent vesicles, and their fusion with vacuoles/lysosomes. Our review will mainly focus on the yeast Saccharomyces cerevisiae, which has been the leading model system for the study of molecular events in autophagy and has led to the discovery of the major mechanistic concepts, which have been found to be mostly conserved in higher eukaryotes.

KW - Atg proteins

KW - PAS

KW - autophagy

KW - dephosphorylation

KW - phagophore

KW - Autophagosomes/metabolism

KW - Vacuoles/metabolism

KW - Autophagy/genetics

KW - Autophagy-Related Proteins/genetics

KW - Saccharomyces cerevisiae Proteins/genetics

KW - Saccharomyces cerevisiae/genetics

KW - Macroautophagy

U2 - 10.1002/1873-3468.14720

DO - 10.1002/1873-3468.14720

M3 - Review

C2 - 37585559

SN - 0014-5793

VL - 598

SP - 73

EP - 83

JO - FEBS Letters

JF - FEBS Letters

IS - 1

ER -