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Peptide-Directed DNA-Templated Protein Labelling for The Assembly of a Pseudo-IgM

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  • Thorbjørn Birger Nielsen, Novo Nordisk A/S
  • ,
  • Rasmus Peter Thomsen
  • ,
  • Michael Rosholm Mortensen
  • ,
  • Jørgen Kjems
  • Per Franklin Nielsen, Novo Nordisk A/S, Denmark
  • Thomas E. Nielsen, Novo Nordisk A/S, Denmark
  • Anne Louise Bank Kodal, Novo Nordisk A/S
  • ,
  • Emiliano Cló, Novo Nordisk A/S, Denmark
  • Kurt Vesterager Gothelf

The development of methods for conjugation of DNA to proteins is of high relevance for the integration of protein function and DNA structures. Here, we demonstrate that protein-binding peptides can direct a DNA-templated reaction, selectively furnishing DNA–protein conjugates with one DNA label. Quantitative conversion of oligonucleotides is achieved at low stoichiometries and the reaction can be performed in complex biological matrixes, such as cell lysates. Further, we have used a star-like pentameric DNA nanostructure to assemble five DNA–Rituximab conjugates, made by our reported method, into a pseudo-IgM antibody structure that was subsequently characterized by negative-stain transmission electron microscopy (nsTEM) analysis.

Original languageEnglish
JournalAngewandte Chemie International Edition
Pages (from-to)9068-9072
Number of pages5
Publication statusPublished - Jul 2019

    Research areas

  • DNA, antibodies, bioconjugation, cyclic peptides, nanostructure

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