Peptide-Directed DNA-Templated Protein Labelling for The Assembly of a Pseudo-IgM

Thorbjørn Birger Nielsen; Rasmus Peter Thomsen; Michael Rosholm Mortensen; Jørgen Kjems; Per Franklin Nielsen; Thomas E.  Nielsen; Anne Louise Bank Kodal; Emiliano Cló; Kurt Vesterager Gothelf

doi:10.1002/anie.201903134

Peptide-Directed DNA-Templated Protein Labelling for The Assembly of a Pseudo-IgM

Thorbjørn Birger Nielsen, Rasmus Peter Thomsen, Michael Rosholm Mortensen, Jørgen Kjems, Per Franklin Nielsen, Thomas E. Nielsen, Anne Louise Bank Kodal, Emiliano Cló, Kurt Vesterager Gothelf

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

30 Citations (Scopus)

Abstract

The development of methods for conjugation of DNA to proteins is of high relevance for the integration of protein function and DNA structures. Here, we demonstrate that protein-binding peptides can direct a DNA-templated reaction, selectively furnishing DNA–protein conjugates with one DNA label. Quantitative conversion of oligonucleotides is achieved at low stoichiometries and the reaction can be performed in complex biological matrixes, such as cell lysates. Further, we have used a star-like pentameric DNA nanostructure to assemble five DNA–Rituximab conjugates, made by our reported method, into a pseudo-IgM antibody structure that was subsequently characterized by negative-stain transmission electron microscopy (nsTEM) analysis.

Original language	English
Journal	Angewandte Chemie International Edition
Volume	58
Issue	27
Pages (from-to)	9068-9072
Number of pages	5
ISSN	1433-7851
DOIs	https://doi.org/10.1002/anie.201903134
Publication status	Published - Jul 2019

Keywords

DNA
antibodies
bioconjugation
cyclic peptides
nanostructure

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10.1002/anie.201903134

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title = "Peptide-Directed DNA-Templated Protein Labelling for The Assembly of a Pseudo-IgM",

abstract = "The development of methods for conjugation of DNA to proteins is of high relevance for the integration of protein function and DNA structures. Here, we demonstrate that protein-binding peptides can direct a DNA-templated reaction, selectively furnishing DNA–protein conjugates with one DNA label. Quantitative conversion of oligonucleotides is achieved at low stoichiometries and the reaction can be performed in complex biological matrixes, such as cell lysates. Further, we have used a star-like pentameric DNA nanostructure to assemble five DNA–Rituximab conjugates, made by our reported method, into a pseudo-IgM antibody structure that was subsequently characterized by negative-stain transmission electron microscopy (nsTEM) analysis.",

keywords = "DNA, antibodies, bioconjugation, cyclic peptides, nanostructure",

author = "Nielsen, {Thorbj{\o}rn Birger} and Thomsen, {Rasmus Peter} and Mortensen, {Michael Rosholm} and J{\o}rgen Kjems and Nielsen, {Per Franklin} and Nielsen, {Thomas E.} and Kodal, {Anne Louise Bank} and Emiliano Cl{\'o} and Gothelf, {Kurt Vesterager}",

year = "2019",

month = jul,

doi = "10.1002/anie.201903134",

language = "English",

volume = "58",

pages = "9068--9072",

journal = "Angewandte Chemie International Edition",

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publisher = "John Wiley and Sons Ltd",

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Peptide-Directed DNA-Templated Protein Labelling for The Assembly of a Pseudo-IgM. / Nielsen, Thorbjørn Birger; Thomsen, Rasmus Peter; Mortensen, Michael Rosholm et al.
In: Angewandte Chemie International Edition, Vol. 58, No. 27, 07.2019, p. 9068-9072.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

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T1 - Peptide-Directed DNA-Templated Protein Labelling for The Assembly of a Pseudo-IgM

AU - Nielsen, Thorbjørn Birger

AU - Thomsen, Rasmus Peter

AU - Mortensen, Michael Rosholm

AU - Kjems, Jørgen

AU - Nielsen, Per Franklin

AU - Nielsen, Thomas E.

AU - Kodal, Anne Louise Bank

AU - Cló, Emiliano

AU - Gothelf, Kurt Vesterager

PY - 2019/7

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N2 - The development of methods for conjugation of DNA to proteins is of high relevance for the integration of protein function and DNA structures. Here, we demonstrate that protein-binding peptides can direct a DNA-templated reaction, selectively furnishing DNA–protein conjugates with one DNA label. Quantitative conversion of oligonucleotides is achieved at low stoichiometries and the reaction can be performed in complex biological matrixes, such as cell lysates. Further, we have used a star-like pentameric DNA nanostructure to assemble five DNA–Rituximab conjugates, made by our reported method, into a pseudo-IgM antibody structure that was subsequently characterized by negative-stain transmission electron microscopy (nsTEM) analysis.

AB - The development of methods for conjugation of DNA to proteins is of high relevance for the integration of protein function and DNA structures. Here, we demonstrate that protein-binding peptides can direct a DNA-templated reaction, selectively furnishing DNA–protein conjugates with one DNA label. Quantitative conversion of oligonucleotides is achieved at low stoichiometries and the reaction can be performed in complex biological matrixes, such as cell lysates. Further, we have used a star-like pentameric DNA nanostructure to assemble five DNA–Rituximab conjugates, made by our reported method, into a pseudo-IgM antibody structure that was subsequently characterized by negative-stain transmission electron microscopy (nsTEM) analysis.

KW - DNA

KW - antibodies

KW - bioconjugation

KW - cyclic peptides

KW - nanostructure

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JO - Angewandte Chemie International Edition

JF - Angewandte Chemie International Edition

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ER -

Peptide-Directed DNA-Templated Protein Labelling for The Assembly of a Pseudo-IgM

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Keywords

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