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Paris-DÉCOR: A Protocol for the Determination of Fast Protein Backbone Amide Hydrogen Exchange Rates

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Standard

Paris-DÉCOR : A Protocol for the Determination of Fast Protein Backbone Amide Hydrogen Exchange Rates. / Dass, Rupashree; Mulder, Frans A.A.

Intrinsically Disordered Proteins. ed. / Birthe B. Kragelund; Karen Skriver. New York : Humana Press, 2020. p. 337-344 (Methods in Molecular Biology, Vol. 2141).

Research output: Contribution to book/anthology/report/proceedingBook chapterResearchpeer-review

Harvard

Dass, R & Mulder, FAA 2020, Paris-DÉCOR: A Protocol for the Determination of Fast Protein Backbone Amide Hydrogen Exchange Rates. in BB Kragelund & K Skriver (eds), Intrinsically Disordered Proteins. Humana Press, New York, Methods in Molecular Biology, vol. 2141, pp. 337-344. https://doi.org/10.1007/978-1-0716-0524-0_17

APA

Dass, R., & Mulder, F. A. A. (2020). Paris-DÉCOR: A Protocol for the Determination of Fast Protein Backbone Amide Hydrogen Exchange Rates. In B. B. Kragelund, & K. Skriver (Eds.), Intrinsically Disordered Proteins (pp. 337-344). Humana Press. Methods in Molecular Biology Vol. 2141 https://doi.org/10.1007/978-1-0716-0524-0_17

CBE

Dass R, Mulder FAA. 2020. Paris-DÉCOR: A Protocol for the Determination of Fast Protein Backbone Amide Hydrogen Exchange Rates. Kragelund BB, Skriver K, editors. In Intrinsically Disordered Proteins. New York: Humana Press. pp. 337-344. (Methods in Molecular Biology, Vol. 2141). https://doi.org/10.1007/978-1-0716-0524-0_17

MLA

Dass, Rupashree and Frans A.A. Mulder "Paris-DÉCOR: A Protocol for the Determination of Fast Protein Backbone Amide Hydrogen Exchange Rates". and Kragelund, Birthe B. Skriver, Karen (editors). Intrinsically Disordered Proteins. New York: Humana Press. (Methods in Molecular Biology, Vol. 2141). 2020, 337-344. https://doi.org/10.1007/978-1-0716-0524-0_17

Vancouver

Dass R, Mulder FAA. Paris-DÉCOR: A Protocol for the Determination of Fast Protein Backbone Amide Hydrogen Exchange Rates. In Kragelund BB, Skriver K, editors, Intrinsically Disordered Proteins. New York: Humana Press. 2020. p. 337-344. (Methods in Molecular Biology, Vol. 2141). https://doi.org/10.1007/978-1-0716-0524-0_17

Author

Dass, Rupashree ; Mulder, Frans A.A. / Paris-DÉCOR : A Protocol for the Determination of Fast Protein Backbone Amide Hydrogen Exchange Rates. Intrinsically Disordered Proteins. editor / Birthe B. Kragelund ; Karen Skriver. New York : Humana Press, 2020. pp. 337-344 (Methods in Molecular Biology, Vol. 2141).

Bibtex

@inbook{ddb34d126e334b62af9182e0a5e59f86,
title = "Paris-D{\'E}COR: A Protocol for the Determination of Fast Protein Backbone Amide Hydrogen Exchange Rates",
abstract = "Determining hydrogen exchange kinetics in proteins can shed light on their structure and dynamics. Nuclear magnetic resonance (NMR) spectroscopy is an important analytical technique to determine exchange rates. In this chapter, we describe a new method (Paris-D{\'E}COR) to determine fast protein amide backbone hydrogen exchange rates in the range 10 to 104 s-1. Measuring fast exchange rates is particularly important for the study of intrinsically disordered proteins, where there is very little protection from exchange to the solvent by the formation of persistent structure. We provide a protocol to set up the experiment as well as MATLAB scripts for numerical simulation that is needed to determine the exchange rates.",
keywords = "Hydrogen exchange, Intrinsically disordered proteins, NMR, Proteins, Spin-spin decorrelation",
author = "Rupashree Dass and Mulder, {Frans A.A.}",
year = "2020",
doi = "10.1007/978-1-0716-0524-0_17",
language = "English",
isbn = "978-1-0716-0523-3",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "337--344",
editor = "Kragelund, {Birthe B.} and Karen Skriver",
booktitle = "Intrinsically Disordered Proteins",

}

RIS

TY - CHAP

T1 - Paris-DÉCOR

T2 - A Protocol for the Determination of Fast Protein Backbone Amide Hydrogen Exchange Rates

AU - Dass, Rupashree

AU - Mulder, Frans A.A.

PY - 2020

Y1 - 2020

N2 - Determining hydrogen exchange kinetics in proteins can shed light on their structure and dynamics. Nuclear magnetic resonance (NMR) spectroscopy is an important analytical technique to determine exchange rates. In this chapter, we describe a new method (Paris-DÉCOR) to determine fast protein amide backbone hydrogen exchange rates in the range 10 to 104 s-1. Measuring fast exchange rates is particularly important for the study of intrinsically disordered proteins, where there is very little protection from exchange to the solvent by the formation of persistent structure. We provide a protocol to set up the experiment as well as MATLAB scripts for numerical simulation that is needed to determine the exchange rates.

AB - Determining hydrogen exchange kinetics in proteins can shed light on their structure and dynamics. Nuclear magnetic resonance (NMR) spectroscopy is an important analytical technique to determine exchange rates. In this chapter, we describe a new method (Paris-DÉCOR) to determine fast protein amide backbone hydrogen exchange rates in the range 10 to 104 s-1. Measuring fast exchange rates is particularly important for the study of intrinsically disordered proteins, where there is very little protection from exchange to the solvent by the formation of persistent structure. We provide a protocol to set up the experiment as well as MATLAB scripts for numerical simulation that is needed to determine the exchange rates.

KW - Hydrogen exchange

KW - Intrinsically disordered proteins

KW - NMR

KW - Proteins

KW - Spin-spin decorrelation

UR - http://www.scopus.com/inward/record.url?scp=85088502942&partnerID=8YFLogxK

U2 - 10.1007/978-1-0716-0524-0_17

DO - 10.1007/978-1-0716-0524-0_17

M3 - Book chapter

C2 - 32696366

AN - SCOPUS:85088502942

SN - 978-1-0716-0523-3

T3 - Methods in Molecular Biology

SP - 337

EP - 344

BT - Intrinsically Disordered Proteins

A2 - Kragelund, Birthe B.

A2 - Skriver, Karen

PB - Humana Press

CY - New York

ER -