Aarhus University Seal

Osteopontin Stabilizes Metastable States Prior to Nucleation during Apatite Formation

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

  • Casper Jon Steenberg Ibsen
  • ,
  • Denis Gebauer, University of Konstanz
  • ,
  • Henrik Birkedal

Osteopontin, which is a phosphoprotein with strong ties to in vivo bone mineralization, is shown to change the precipitation pathway of calcium phosphate. We show that the presence of the phosphoprotein, even in minute concentrations, can stabilize an otherwise oversaturated mixture against precipitation. At moderate concentrations, we find that the protein introduces a new intermediate state into the reaction pathway leading to apatite formation. This new intermediate was found to share many characteristics of a coacervate or polymer-induced liquid-like precursor (PILP) phase. Our results show that these types of complex phases should be considered when discussing the mechanisms of bone mineralization on a subcellular level.

Original languageEnglish
JournalChemistry of Materials
Pages (from-to)8550-8555
Number of pages6
Publication statusPublished - 13 Dec 2016

See relations at Aarhus University Citationformats

ID: 107830503